The Effect of In Ovo Administration of Rosemary Essential Oil on Hatchability, Relative Hatching Weight, and Embryo Mortality Rate in Japanese Quail (<i>Coturnix coturnix japonica</i>)
Assia Aberbour,
Leghel Touazi,
Amine Benberkane,
Sofiane Aissanou,
Anjum Sherasiya,
Mokrane Iguer-Ouada,
Jean Luc Hornick,
Nassim Moula
Affiliations
Assia Aberbour
Department of Veterinary Management of Animal Resources, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium
Leghel Touazi
Department of Agronomy, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif, EL Bez, Setif 19000, Algeria
Amine Benberkane
Associated Laboratory in Marine Ecosystems and Aquaculture, Department of Biological Sciences of the Environment, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria
Sofiane Aissanou
Associated Laboratory in Marine Ecosystems and Aquaculture, Department of Biological Sciences of the Environment, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria
Anjum Sherasiya
Veterinary World, Star, Gulshan Park, NH-8A, Chandrapur Road, Wankaner 363621, India
Mokrane Iguer-Ouada
Associated Laboratory in Marine Ecosystems and Aquaculture, Department of Biological Sciences of the Environment, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria
Jean Luc Hornick
Department of Veterinary Management of Animal Resources, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium
Nassim Moula
Department of Veterinary Management of Animal Resources, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium
This study aimed to determine the effects of air sac injection of rosemary essential oil at different concentrations in ovo in quail eggs on hatching rate, relative chick weight at hatching, and embryonic mortality rate. A total of 1060 Japanese quail eggs were divided into four groups: negative control (non-injected), positive control (30 µL sterile distilled water/egg), and two treated groups with 1 and 3 µL oil/egg, respectively. The concentration of 3 µL/egg showed a toxic effect on embryonic development, as revealed by the significantly (p = 0.015) higher post-injection mortality rate (18.21%) compared to 1 µL/egg with 8.3%. Furthermore, hatchability was significantly increased (p = 0.0001) with 1 µL/egg compared to 3 µL/egg with 69.1% and 44.48%, respectively. No significant difference was observed between the concentration of 1 µL/egg and the control groups (p = 0.822). Both l and 3 µL essential oil/egg significantly enhanced (p = 0.0001) relative chick weight at hatching by 67.14% and 70.32%, respectively, compared to the control groups. In conclusion, injecting eggs with 1 µL oil/egg showed positive effects both on hatching and relative chick weight. The concentration of 3 µL/egg was revealed to be toxic, with dramatic effects on embryonic survival.
