Detection of drug resistance in Escherichia coli from calves with diarrhea in the Tongliao region: an analysis of multidrug-resistant strains

Zi Wang; Zi Wang; Zi Wang; Miao Sun; Shuang Guo; Yongqiang Wang; Linghao Meng; Jinchuan Shi; Chao Geng; Dongxu Han; Dongxu Han; Dongxu Han; Xiaomeng Fu; Jiangdong Xue; Jiangdong Xue; Jiangdong Xue; Hongxia Ma; Kai Liu; Kai Liu; Kai Liu

doi:10.3389/fvets.2024.1466690

Frontiers in Veterinary Science (Nov 2024)

Detection of drug resistance in Escherichia coli from calves with diarrhea in the Tongliao region: an analysis of multidrug-resistant strains

Zi Wang,
Zi Wang,
Zi Wang,
Miao Sun,
Shuang Guo,
Yongqiang Wang,
Linghao Meng,
Jinchuan Shi,
Chao Geng,
Dongxu Han,
Dongxu Han,
Dongxu Han,
Xiaomeng Fu,
Jiangdong Xue,
Jiangdong Xue,
Jiangdong Xue,
Hongxia Ma,
Kai Liu,
Kai Liu,
Kai Liu

Affiliations

Zi Wang: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China
Zi Wang: Inner Mongolia Engineering Technology Research Center for Prevention and Control of Beef Cattle Diseases, Tongliao, China
Zi Wang: Beef Cattle Industry School of Inner Mongolia Autonomous Region, Tongliao, China
Miao Sun: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China
Shuang Guo: Hinggan League Animal Disease Control Center, Hinggan League, China
Yongqiang Wang: Zhalantun Vocational College, Hulunbeier, China
Linghao Meng: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China
Jinchuan Shi: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China
Chao Geng: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China
Dongxu Han: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China
Dongxu Han: Inner Mongolia Engineering Technology Research Center for Prevention and Control of Beef Cattle Diseases, Tongliao, China
Dongxu Han: Beef Cattle Industry School of Inner Mongolia Autonomous Region, Tongliao, China
Xiaomeng Fu: Tongliao Vocational College, Tongliao, China
Jiangdong Xue: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China
Jiangdong Xue: Inner Mongolia Engineering Technology Research Center for Prevention and Control of Beef Cattle Diseases, Tongliao, China
Jiangdong Xue: Beef Cattle Industry School of Inner Mongolia Autonomous Region, Tongliao, China
Hongxia Ma: College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
Kai Liu: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China
Kai Liu: Inner Mongolia Engineering Technology Research Center for Prevention and Control of Beef Cattle Diseases, Tongliao, China
Kai Liu: Beef Cattle Industry School of Inner Mongolia Autonomous Region, Tongliao, China

DOI: https://doi.org/10.3389/fvets.2024.1466690
Journal volume & issue: Vol. 11

Abstract

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IntroductionEscherichia coli is a major pathogen responsible for calf diarrhea, which has been exacerbated by the irrational and unscientific use of antimicrobial drugs, leading to significant drug resistance.MethodsThis study focused on the isolation and identification of E. coli from calf diarrhea samples in the Tongliao area of China. Isolation was conducted using selective media, Gram staining, and 16S rRNA sequencing. The minimum inhibitory concentration (MIC) of E. coli was determined through the microbroth dilution method. Additionally, the presence of antibiotic-resistant genes was detected, and multidrug-resistant strains were selected for whole-genome sequencing (WGS).ResultsThe results revealed that all 40 isolated strains of E. coli exhibited resistance to sulfadiazine sodium, enrofloxacin, and ciprofloxacin, with 90% of the strains being susceptible to polymyxin B. Notably, strains 11, 23, and 24 demonstrated severe resistance. The detection rates of the antibiotic resistance genes TEM-1, TEM-206, strA, strB, qacH, and blaCTX were 100%, indicating a high prevalence of these genes. Moreover, the majority of strains carried antibiotic resistance genes consistent with their resistance phenotypes. WGS of strains 11, 23, and 24 revealed genome sizes of 4,897,185 bp, 4,920,234 bp, and 4,912,320 bp, respectively. These strains carried two, one, and two plasmids, respectively. The prediction of antibiotic resistance genes showed a substantial number of these genes within the genomes, with strain 24 harboring the highest number, totaling 77 subspecies containing 88 antibiotic resistance genes.DiscussionIn conclusion, all 40 isolated strains of E. coli from calf diarrhea in this study were multidrug-resistant, exhibiting a broad distribution of antibiotic resistance genes and mobile components. This poses a significant risk of horizontal gene transfer, highlighting the critical situation of antibiotic resistance in this region.

Published in Frontiers in Veterinary Science

ISSN: 2297-1769 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: https://www.frontiersin.org/journals/veterinary-science

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