Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferation

Abstract

Pathological conditions such as infections, cancer, inflammation, or iatrogenic lesions, can hinder the correct functionality of the ureter and its structural integrity. Several strategies for the regeneration of the ureter and the restoration of its functionality are available but the best strategy has not been reached yet. Among the proposed strategies, a promising one is the development of tubular scaffolds, in particular exploiting electrospinning technique. In this work, antibacterial electrospun polycaprolactone/rifampicin (PCL/Rif) membranes were prepared and characterized. The membranes are characterized by randomly oriented nanofibers with a homogeneous diameter, as determined by scanning electron microscopy. The mechanical characterization, performed with uniaxial tensile tests, showed a suitable stability over time and a proper deformability. The rifampicin release, investigated by UV spectrophotometry, showed a burst release in the first part of the experiment and a sustained release over time. The membranes are biocompatible and able to support the adhesion and proliferation of urotheliocytes. Moreover, PCL/Rif membranes showed an antibacterial activity against Escherichia coli and other bacterial strains belonging to the “ESKAPE” group. Considering the possibility to use the electrospinning for the production of tubular scaffolds, the described membranes represent a promising starting point for the preparation of ureteral scaffolds with antibacterial properties.

Keywords

• Antibacterial
• Biomaterial
• Electrospinning
• Polycaprolactone
• Rifampicin
• Ureteral regeneration