Flanking sequences influence the activity of TET1 and TET2 methylcytosine dioxygenases and affect genomic 5hmC patterns

Sabrina Adam; Julia Bräcker; Viviane Klingel; Bernd Osteresch; Nicole E. Radde; Jens Brockmeyer; Pavel Bashtrykov; Albert Jeltsch

doi:10.1038/s42003-022-03033-4

Communications Biology (Jan 2022)

Flanking sequences influence the activity of TET1 and TET2 methylcytosine dioxygenases and affect genomic 5hmC patterns

Sabrina Adam,
Julia Bräcker,
Viviane Klingel,
Bernd Osteresch,
Nicole E. Radde,
Jens Brockmeyer,
Pavel Bashtrykov,
Albert Jeltsch

Affiliations

Sabrina Adam: Institute of Biochemistry and Technical Biochemistry, Department of Biochemistry, University of Stuttgart
Julia Bräcker: Institute of Biochemistry and Technical Biochemistry, Department of Food Chemistry, University of Stuttgart
Viviane Klingel: Institute for Systems Theory and Automatic Control, University of Stuttgart
Bernd Osteresch: Institute of Biochemistry and Technical Biochemistry, Department of Food Chemistry, University of Stuttgart
Nicole E. Radde: Institute for Systems Theory and Automatic Control, University of Stuttgart
Jens Brockmeyer: Institute of Biochemistry and Technical Biochemistry, Department of Food Chemistry, University of Stuttgart
Pavel Bashtrykov: Institute of Biochemistry and Technical Biochemistry, Department of Biochemistry, University of Stuttgart
Albert Jeltsch: Institute of Biochemistry and Technical Biochemistry, Department of Biochemistry, University of Stuttgart

DOI: https://doi.org/10.1038/s42003-022-03033-4
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 13

Abstract

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Sabrina Adam et al. use a deep enzymology method to study the effect of neighboring DNA sequence variation on the in vitro activity of Tet1 and Tet2. Their results suggest that flanking sequences could represent an important parameter that influences genomic DNA modification patterns.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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