OncoTargets and Therapy (Jul 2020)

Upregulation of FTX Promotes Osteosarcoma Tumorigenesis by Increasing SOX4 Expression via miR-214-5p

  • Chen H,
  • Liu T,
  • Ouyang H,
  • Lin S,
  • Zhong H,
  • Zhang H,
  • Yang Y

Journal volume & issue
Vol. Volume 13
pp. 7125 – 7136

Abstract

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Haicong Chen,1,* Tianfeng Liu,1,* Hanbin Ouyang,1 Sien Lin,1 Huan Zhong,1 Hongwu Zhang,2 Yang Yang2 1Department of Orthopedics Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, Guangdong, People’s Republic of China; 2Department of Anatomy, School of Basic Medicine Science, Southern Medical University, Guangzhou 510515, Guangdong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Huan ZhongDepartment of Orthopedics Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, Guangdong, People’s Republic of ChinaTel +86 759-2369606Email [email protected]: Long-chain non-coding RNA (LncRNA) plays a key role in the biological processes of tumors. LncRNA-FTX has been the invasion of tumors. However, its function and mechanism in osteosarcoma have not been studied.Methods: qRT-PCR was measured the expression levels of FTX and miR-214-5p in osteosarcoma. The protein levels of SRY-related HMG box transcription factor 4 (SOX4) were detected by Western Blot. Cholecystokinin (CCK-8) assay, cell colony formation and Transwell assay, Annexin V-FITC/PI assay were analyzed the effects of FTX and miR-214-5p on cell proliferation, cell invasion and apoptosis. The relationship between FTX, miR-214-5p and SOX4 was analyzed by bioinformatics analysis and Luciferase. The tumor changes in mice were detected by vivo experiments in nude mice.Results: The expression levels of FTX were increased in osteosarcoma tissues and cell lines and negatively correlated with the expression levels of miR-214-5p. FTX could modulate the expression of miR-214-5p in osteosarcoma cell lines. sh-FTX inhibited the growth and metastasis of osteosarcoma. FTX could regulate the growth of osteosarcoma through miR-214-5p. The knockdown of miR-214-5p reversed the inhibitory effect of sh-FTX on osteosarcoma cell proliferation and growth in mice. Furthermore, FTX regulated the expression of SOX4 by acting as a sponge of miR-214-5p in osteosarcoma.Conclusion: FTX could promote proliferation, invasion and inhibited apoptosis by regulating miR-214-5p/SOX4 axis in osteosarcoma, suggesting that FTX might be a potential target for osteosarcoma treatment.Keywords: FTX, miR-214-5p, SOX4, osteosarcoma, proliferation, apoptosis

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