Boron Supply Enhances Aluminum Tolerance in Root Border Cells of Pea (Pisum sativum) by Interacting with Cell Wall Pectins

Xue Wen Li; Jia You Liu; Jing Fang; Jing Fang; Lin Tao; Lin Tao; Ren Fang Shen; Ya Lin Li; Ya Lin Li; Hong Dong Xiao; Ying Ming Feng; Hai Xiang Wen; Jia Hua Guan; Li Shu Wu; Yong Ming He; Heiner E. Goldbach; Min Yu

doi:10.3389/fpls.2017.00742

Frontiers in Plant Science (May 2017)

Boron Supply Enhances Aluminum Tolerance in Root Border Cells of Pea (Pisum sativum) by Interacting with Cell Wall Pectins

Xue Wen Li,
Jia You Liu,
Jing Fang,
Jing Fang,
Lin Tao,
Lin Tao,
Ren Fang Shen,
Ya Lin Li,
Ya Lin Li,
Hong Dong Xiao,
Ying Ming Feng,
Hai Xiang Wen,
Jia Hua Guan,
Li Shu Wu,
Yong Ming He,
Heiner E. Goldbach,
Min Yu

Affiliations

Xue Wen Li: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China
Jia You Liu: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China
Jing Fang: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China
Jing Fang: College of Resources and Environment, Huazhong Agricultural UniversityWuhan, China
Lin Tao: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China
Lin Tao: College of Resources and Environment, Huazhong Agricultural UniversityWuhan, China
Ren Fang Shen: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesNanjing, China
Ya Lin Li: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China
Ya Lin Li: College of Resources and Environment, Huazhong Agricultural UniversityWuhan, China
Hong Dong Xiao: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China
Ying Ming Feng: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China
Hai Xiang Wen: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China
Jia Hua Guan: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China
Li Shu Wu: College of Resources and Environment, Huazhong Agricultural UniversityWuhan, China
Yong Ming He: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China
Heiner E. Goldbach: Plant Nutrition-Institute of Crop Science and Resource Conservation, University of BonnBonn, Germany
Min Yu: Department of Horticulture, School of Food Science and Engineering, School of Life Science and Engineering, Foshan UniversityGuangdong, China

DOI: https://doi.org/10.3389/fpls.2017.00742
Journal volume & issue: Vol. 8

Abstract

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Aluminum (Al) toxicity is the primary factor limiting crop growth in acidic soils. Boron (B) alleviates Al toxicity in plants, which is mainly considered to be due to the formation of Rhamnogalacturonan II-B (RGII-B) complexes, which helps to stabilize the cytoskeleton. It is unclear yet whether this is due to the increasing of net negative charges and/or further mechanisms. Kinetics of Al accumulation and adsorption were investigated using entire cells, cell wall and pectin of root border cells (RBCs) of pea (Pisum sativum), to reveal the mechanism of B in interacting with alkali-soluble and chelator-soluble pectin for an increased Al tolerance in RBCs. The results show that B could rescue RBCs from Al-induced cell death by accumulating more Al in the cell wall, predominately in alkali-soluble pectin. Boron also promotes Al3+ adsorption and inhibits Al3+ desorption from alkali-soluble pectin. Thus, more Al3+ is immobilized within the alkali-soluble pectin fraction and less in the chelator-soluble pectin, rendering Al3+ less mobile. Boron induces an increase of RG-II (KDO,2-keto-3-deoxyoctonic acid) content for forming more borate-RGII complexes, and the decrease of pectin methyl-esterification, thus creates more negative charges to immobilize Al3+ in cell wall pectin. The study provides evidence that abundant B supply enhances the immobilization of Al in alkali-soluble pectin, thus most likely reducing the entry of Al3+ into the symplast from the surroundings.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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