Di-san junyi daxue xuebao (Aug 2019)

MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene

  • HAO Junjian,
  • HAO Lijuan

DOI
https://doi.org/10.16016/j.1000-5404.201903113
Journal volume & issue
Vol. 41, no. 16
pp. 1559 – 1565

Abstract

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Objective To investigate the effects of miR-204-5p on the proliferation, invasion and migration of thyroid papillary carcinoma K1 cells and its mechanism. Methods K1 cells cultured in vitro were transfected with a miR-204-5p mimics, a miR-204-5p inhibitor, or their respective negative controls (miR-NC and anti-miR-NC), and the expression of miR-204-5p in the cells was detected using RT-PCR. The changes in cell proliferation were assessed with MTT assay, and the changes in cell invasion and migration were evaluated with Transwell assay. The targeting relationship between miR-204-5p and HMGB1 gene was tested using dual luciferase reporter gene assay, and the regulation of miR-204-5p on the expressions of HMGB1 mRNA and protein was examined using RT-PCR and Western blotting. The changes in the proliferation, invasion and migration of K1 cells were observed after transfection of the cells with siRNA-HMGB1 or a HMGB1 overexpression plasmid. Results The expression level of miR-204-5p was significantly increased in the cells transfected with miR-204-5p mimics and significantly lowered in cells transfected with miR-204-5p inhibitor as compared with their respective controls (P < 0.05). Overexpression of miR-204-5p significantly attenuated while inhibition of miR-204-5p significantly enhanced the proliferation, invasion and migration abilities of the cells in comparison with their respective controls (P < 0.05). HMGB1 was a potential target gene of miR-204-5p, and was negatively regulated by miR-204-5p. RNA interference of HMGB1 obviously inhibited K1 cell proliferation, invasion and migration, while overexpression of HMGB1 promoted the proliferation, invasion and migration of the cells. Conclusion Overexpression of miR-204-5p can inhibit the proliferation, invasion and migration of K1 cells by targeted down-regulation of HMGB1 expression.

Keywords