Armaghane Danesh Bimonthly Journal (Mar 2021)
Cytotoxic Effect of Hydroalcoholic Extract of Stachys setifera on MCF-7 Human Breast Cancer Cell Line
Abstract
Background & aim: Breast cancer is the most common cancer among women. Today, compounds derived from medicinal plants are used in the treatment of various cancers. Despite reports of antitumor effects on some Stachys species, the activity of Stachys setifera extract on breast cancer cell lines has not been studied. Therefore, the aim of the present study was to investigate the effect of hydroalcoholic extract of Stachys setifera on MCF-7 cancer cell proliferation. Methods: In the present experimental study conducted in 2019, after culturing MCF-7 cancer cells, the cells were incubated with different concentrations Stachys setifera extract and cisplatin (as positive control) for 24 hours. Cancer cell survival and inhibitory concentration of 50% (IC50) were assessed using MTT colorimetric method. The effects of extract and cisplatin on cell survival and cell cycle were evaluated by flow cytometry. The collected data were analyzed using one-way analysis of variance and Tukey's post hoc test. The significance level was considered p <0.05. Results: The IC50 results of hydroalcoholic extract of Stachys setifera and cisplatin on MCF-7 cells after 24 hours were 827.52 and 4.34 μg / ml, respectively. Flow cytometry results showed that Stachys setifera extract and cisplatin stopped the growth of MCF-7 cells in G0 / G1 phase and the percentage of cells in control cells, extract and cisplatin (42.94, 50.68 and 59.11), respectively. The Stachys setifera extract also caused some morphological changes such as granulation of the cytoplasm and rounding and enlargement of cells in the MCF-7 cancer cell Conclusion: The results indicated that Stachys setifera hydroalcoholic extract inhibited the proliferation of MCF-7 breast cancer cells and was able to exert its cytotoxic effects by affecting cell cycle and induce morphological changes. Therefore, this plant can be a suitable candidate to purify its main compounds for further study on proliferation of breast cancer cells. The results also revealed that the hydroalcoholic extract of Stachys setifera inhibited the proliferation of MCF-7 breast cancer cells and was able to enter the cells in the apoptotic phase. Therefore, this plant can be a suitable candidate for further study to prevent the proliferation of breast cancer.
