Catalytic cleavage activities of 10–23 DNAzyme analogs functionalized with an amino group in its catalytic core

Abstract

Functionalization of the catalytic loop of 10–23 DNAzyme with an amino group was performed by incorporation of 7-(3-aminopropyl)-8-aza-7-deaza-2′-deoxyadenosine in different single positions. Among the nine modified positions in the catalytic loop, A9 is the unique position with positive contribution by such modification. These results indicated that more efficient deoxyribozymes remain to be explored by introduction of exogenous functional groups in an appropriate position in the catalytic loop of 10–23 DNAzyme, such as the combination of 7-functional group substituted 8-aza-7-deaza-2′-deoxyadenosine analogs and A9 position.

Keywords

• 10–23 DNAzyme
• 8-Aza-7-deaza-2′-deoxyadenosine
• Amino group
• Chemical modification