Evaluation of the In Vitro Behavior of Electrochemically Deposited Plate-like Crystal Hydroxyapatite Coatings
Cosmin M. Cotrut,
Alexandru Blidisel,
Diana M. Vranceanu,
Alina Vladescu (Dragomir),
Elena Ungureanu,
Iulian Pana,
Mihaela Dinu,
Catalin Vitelaru,
Anca C. Parau,
Vasile Pruna,
Mihai S. Magurean,
Irina Titorencu
Affiliations
Cosmin M. Cotrut
Faculty of Materials Science and Engineering, National University of Science and Technology Politehnica Bucharest, 313 Independentei Street, 060042 Bucharest, Romania
Alexandru Blidisel
Hepato-Bilio-Pancreatic Surgery Center, University Clinic Surgical Semiology and Thoracic Surgery, “Victor Babes” University of Medicine and Pharmacy, Sq. Eftimie Murgu No. 2, 300041 Timisoara, Romania
Diana M. Vranceanu
Faculty of Materials Science and Engineering, National University of Science and Technology Politehnica Bucharest, 313 Independentei Street, 060042 Bucharest, Romania
Alina Vladescu (Dragomir)
Department for Advanced Surface Processing and Analysis by Vacuum Technologies, National Institute of Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, Romania
Elena Ungureanu
Faculty of Materials Science and Engineering, National University of Science and Technology Politehnica Bucharest, 313 Independentei Street, 060042 Bucharest, Romania
Iulian Pana
Department for Advanced Surface Processing and Analysis by Vacuum Technologies, National Institute of Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, Romania
Mihaela Dinu
Department for Advanced Surface Processing and Analysis by Vacuum Technologies, National Institute of Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, Romania
Catalin Vitelaru
Department for Advanced Surface Processing and Analysis by Vacuum Technologies, National Institute of Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, Romania
Anca C. Parau
Department for Advanced Surface Processing and Analysis by Vacuum Technologies, National Institute of Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, Romania
Vasile Pruna
Romanian Academy Institute of Cellular Biology and Pathology “Nicolae Simionescu”, 8 B.P. Hasdeu, 050568 Bucharest, Romania
Mihai S. Magurean
Colentina Clinical Hospital, 020125 Bucharest, Romania
Irina Titorencu
Romanian Academy Institute of Cellular Biology and Pathology “Nicolae Simionescu”, 8 B.P. Hasdeu, 050568 Bucharest, Romania
The purpose of coatings is to protect or enhance the functionality of the substrate material, irrespective of the field in which the material was designed. The use of coatings in medicine is rapidly expanding with the objective of enhancing the osseointegration ability of metallic materials such as titanium. The aim of this study was to obtain biomimetic hydroxyapatite (HAp)-based coatings on titanium by using the pulsed galvanostatic method. The morphology of the HAp-based coatings revealed the presence of very thin and wide plate-like crystals, grown perpendicular to the Ti substrate, while the chemical composition highlighted a Ca/P ratio of 1.66, which is close to that of stoichiometric HAp (1.67). The main phases and chemical bonds identified confirmed the presence of the HAp phase in the developed coatings. A roughness of 228 nm and a contact angle of approx. 17° were obtained for the HAp coatings, highlighting a hydrophilic character. In terms of biomineralization and electrochemical behavior, it was shown that the HAp coatings have significantly enhanced the titanium properties. Finally, the in vitro cell tests carried out with human mesenchymal stem cells showed that the Ti samples coated with HAp have increased cell viability, extracellular matrix, and Ca intracellular deposition when compared with the uncoated Ti, indicating the beneficial effect.
