Learning from Seed Microbes: Trichoderma Coating Intervenes in Rhizosphere Microbiome Assembly

Penghao Xie; Shengdie Yang; Xiaoyu Liu; Tianyi Zhang; Xinyuan Zhao; Tao Wen; Jian Zhang; Chao Xue; Qirong Shen; Jun Yuan

doi:10.1128/spectrum.03097-22

Microbiology Spectrum (Jun 2023)

Learning from Seed Microbes: Trichoderma Coating Intervenes in Rhizosphere Microbiome Assembly

Penghao Xie,
Shengdie Yang,
Xiaoyu Liu,
Tianyi Zhang,
Xinyuan Zhao,
Tao Wen,
Jian Zhang,
Chao Xue,
Qirong Shen,
Jun Yuan

Affiliations

Penghao Xie: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, The Key Laboratory of Plant Immunity, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China
Shengdie Yang: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, The Key Laboratory of Plant Immunity, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China
Xiaoyu Liu: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, The Key Laboratory of Plant Immunity, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China
Tianyi Zhang: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, The Key Laboratory of Plant Immunity, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China
Xinyuan Zhao: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, The Key Laboratory of Plant Immunity, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China
Tao Wen: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, The Key Laboratory of Plant Immunity, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China
Jian Zhang: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, The Key Laboratory of Plant Immunity, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China
Chao Xue: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, The Key Laboratory of Plant Immunity, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China
Qirong Shen: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, The Key Laboratory of Plant Immunity, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China
Jun Yuan: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, The Key Laboratory of Plant Immunity, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China

DOI: https://doi.org/10.1128/spectrum.03097-22
Journal volume & issue: Vol. 11, no. 3

Abstract

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ABSTRACT Seed-associated microbiomes can impact the later colonization of a plant rhizosphere microbiome. However, there remains little insight into the underlying mechanisms concerning how alterations in the composition of the seed microbiome may intervene in the assembly of a rhizosphere microbiome. In this study, the fungus Trichoderma guizhouense NJAU4742 was introduced to both maize and watermelon seed microbiomes by seed coating. Application was found to significantly promote seed germination and improve plant growth and rhizosphere soil quality. The activities of acid phosphatase, cellulase, peroxidase, sucrase, and α-glucosidase increased significantly in two crops. The introduction of Trichoderma guizhouense NJAU4742 also led to a decrease in the occurrence of disease. Coating with T. guizhouense NJAU4742 did not alter the alpha diversities of the bacterial and fungal communities but formed a key network module that contained both Trichoderma and Mortierella. This key network module comprised of these potentially beneficial microorganisms was positively linked with the belowground biomass and activities of rhizosphere soil enzymes but negatively correlated with disease incidence. Overall, this study provides insights into plant growth promotion and plant health maintenance via seed coating in order to influence the rhizosphere microbiome. IMPORTANCE Seed-associated microbiomes can impact the rhizosphere microbiome assembly and function display. However, there remains little insight into the underlying mechanisms concerning how alterations in the composition of the seed microbiome with the beneficial microbes may intervene in the assembly of a rhizosphere microbiome. Here, we introduced T. guizhouense NJAU4742 to the seed microbiome by seed coating. This introduction led to a decrease in the occurrence of disease and an increase in plant growth; furthermore, it formed a key network module that contained both Trichoderma and Mortierella. Our study provides insights into plant growth promotion and plant health maintenance via seed coating in order to influence the rhizosphere microbiome.

Published in Microbiology Spectrum

ISSN: 2165-0497 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/spectrum

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