Biomedical and Biotechnology Research Journal (Jan 2018)

Studies on 3H-levamisole binding to murine splenic lymphocytes, normal, malignant human lymphocytes and fate of levamisole in cell culture

  • B Nageshwari,
  • Ramchander Merugu

DOI
https://doi.org/10.4103/bbrj.bbrj_106_17
Journal volume & issue
Vol. 2, no. 1
pp. 53 – 58

Abstract

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Background: Levamisole at high concentrations has been shown to have anticancer and immunosuppressive actions. Methods: In the present study, fate of levamisole in cell culture and 3 H-levamisole binding to murine splenic lymphocytes, normal and malignant human lymphocytes has been investigated. Results: High-performance liquid chromatography analysis of cell culture supernatants of myeloma cells treated with levamisole has shown that products of levamisole appeared with progressive culture period indicating a metabolic transformation. 3 H-levamisole binding assays indicate that the binding was signifi cantly higher in lysates of lipopolysaccharide-stimulated murine splenic lymphocytes as compared to whole cells. Conclusion: The degradation of levamisole could be one more possible reason for the high concentration of levamisole required to get the desirable cytotoxic effect on myeloma cells.

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