Quantifying Titanium Exposure in Lung Tissues: A Novel Laser‐Induced Breakdown Spectroscopy Elemental Imaging‐Based Analytical Framework for Biomedical Applications
Vincent Gardette,
Lucie Sancey,
Marine Leprince,
Laurent Gaté,
Frederic Cosnier,
Carole Seidel,
Sarah Valentino,
Frederic Pelascini,
Jean‐Luc Coll,
Michel Péoc'h,
Virginie Scolan,
François Paysant,
Vincent Bonneterre,
Christophe Dujardin,
Benoit Busser,
Vincent Motto‐Ros
Affiliations
Vincent Gardette
Université de Lyon Université Claude Bernard Lyon 1 CNRS Institut Lumière Matière Villeurbanne 69100 France
Lucie Sancey
Univ. Grenoble Alpes INSERM U1209 CNRS 5309 Institute for Advanced Biosciences Grenoble 38000 France
Marine Leprince
Université de Lyon Université Claude Bernard Lyon 1 CNRS Institut Lumière Matière Villeurbanne 69100 France
Laurent Gaté
Institut national de recherche et de sécurité pour la prévention des accidents du travail et des maladies professionnelles (INRS) Département Toxicologie et Biométrologie Vandoeuvre‐lès‐Nancy 54519 France
Frederic Cosnier
Institut national de recherche et de sécurité pour la prévention des accidents du travail et des maladies professionnelles (INRS) Département Toxicologie et Biométrologie Vandoeuvre‐lès‐Nancy 54519 France
Carole Seidel
Institut national de recherche et de sécurité pour la prévention des accidents du travail et des maladies professionnelles (INRS) Département Toxicologie et Biométrologie Vandoeuvre‐lès‐Nancy 54519 France
Sarah Valentino
Institut national de recherche et de sécurité pour la prévention des accidents du travail et des maladies professionnelles (INRS) Département Toxicologie et Biométrologie Vandoeuvre‐lès‐Nancy 54519 France
Frederic Pelascini
CETIM Illkirch‐Graffenstaden 67402 France
Jean‐Luc Coll
Univ. Grenoble Alpes INSERM U1209 CNRS 5309 Institute for Advanced Biosciences Grenoble 38000 France
Michel Péoc'h
University Hospital of Grenoble Alpes Grenoble 38000 France
Virginie Scolan
University Hospital of Grenoble Alpes Grenoble 38000 France
François Paysant
University Hospital of Grenoble Alpes Grenoble 38000 France
Vincent Bonneterre
University Hospital of Grenoble Alpes Grenoble 38000 France
Christophe Dujardin
Université de Lyon Université Claude Bernard Lyon 1 CNRS Institut Lumière Matière Villeurbanne 69100 France
Benoit Busser
Univ. Grenoble Alpes INSERM U1209 CNRS 5309 Institute for Advanced Biosciences Grenoble 38000 France
Vincent Motto‐Ros
Université de Lyon Université Claude Bernard Lyon 1 CNRS Institut Lumière Matière Villeurbanne 69100 France
Occupational and environmental exposures, particularly those related to urban and suburban atmospheres, are increasingly linked to a range of pulmonary diseases. While diagnostic methods for these diseases are well established, analytical tools for assessing elemental contamination in lung tissue remain underutilized. This study introduces a novel framework based on laser‐induced breakdown spectroscopy (LIBS) for the in situ quantification of elemental titanium (Ti) in lung tissues from both animal models and human specimens. Rigorous validation is conducted using animal models exposed to TiO2 P25 nanoparticles and a comparative analysis with inductively coupled plasma mass spectrometry. The novel quantitative metric Svalue demonstrates robust correlation with elemental concentrations, expanding LIBS utility to volumetric organ analysis. This validated methodology is subsequently applied to human lung specimens preserved in paraffin. The research holds significant promise as a diagnostic tool for assessing exposure levels to environmental or occupational hazards, thereby offering valuable contributions to the fields of toxicology and respiratory medicine.