Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

Raghuvir Viswanatha; Enzo Mameli; Jonathan Rodiger; Pierre Merckaert; Fabiana Feitosa-Suntheimer; Tonya M. Colpitts; Stephanie E. Mohr; Yanhui Hu; Norbert Perrimon

doi:10.1038/s41467-021-27129-3

Nature Communications (Nov 2021)

Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

Raghuvir Viswanatha,
Enzo Mameli,
Jonathan Rodiger,
Pierre Merckaert,
Fabiana Feitosa-Suntheimer,
Tonya M. Colpitts,
Stephanie E. Mohr,
Yanhui Hu,
Norbert Perrimon

Affiliations

Raghuvir Viswanatha: Department of Genetics, Blavatnik Institute, Harvard Medical School
Enzo Mameli: Department of Genetics, Blavatnik Institute, Harvard Medical School
Jonathan Rodiger: Department of Genetics, Blavatnik Institute, Harvard Medical School
Pierre Merckaert: Department of Genetics, Blavatnik Institute, Harvard Medical School
Fabiana Feitosa-Suntheimer: Department of Microbiology, National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, 620 Albany Street
Tonya M. Colpitts: Department of Microbiology, National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, 620 Albany Street
Stephanie E. Mohr: Department of Genetics, Blavatnik Institute, Harvard Medical School
Yanhui Hu: Department of Genetics, Blavatnik Institute, Harvard Medical School
Norbert Perrimon: Department of Genetics, Blavatnik Institute, Harvard Medical School

DOI: https://doi.org/10.1038/s41467-021-27129-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 13

Abstract

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Forward genetic approaches such as CRISPR screens are powerful ways to identify essential genes and those that influence host-pathogen interactions. Here the authors design a bioinformatics portal for sgRNA design and a recombination-mediated cassette system for delivery into mosquito cell lines.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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