Diagnostic Significance of the Parietal and Cavity Microllora of the Large Intestine in Antibiotic-Associated Dysbacteriosis

Abstract

The study examined the qualitative and quantitative composition of the parietal and cavity bacterial flora of the large intestine under the influence of ceftriaxone in the dynamics of a monthly animal experiment. The study was conducted on Wistar rats, divided into two groups. Control group (n=50): 1.0 ml of saline solution was injected intramuscularly into animals daily for 10 days. Test group (n=50): ceftriaxone 15 mg/kg/day was intramuscularly injected to animals daily for 10 days. Intestinal contents from the distal third of the colon were used to carry out bacteriological analysis. The studied material was put on nutrient media for the isolation of non-fermentative gram-negative bacteria - NGOB (including pseudomonads), enterobacteria, bifidobacteria, lactobacteria, clostridia, escherichia, enterococci, staphylococci, yeast-like fungi. From the diagnostic point of view, the cavity microflora undergoes more pronounced qualitative and quantitative changes, which do not allow extrapolating the results of microbiological analysis of the cavity microflora to the parietal one unequivocally. As it was established in the study, the presence of conditionally pathogenic microorganisms in the abdominal microflora does not indicate the presence of these representatives of the microflora in the parietal layer.

Keywords

• antibacterial therapy
• experiment
• diagnostic significance
• qualitative and quantitative composition of the cavity and parietal microflora of the intestine
• antibacterial therapy
• experiment
• diagnostic significance
• qualitative and quantitative composition of the cavity and parietal microflora of the intestine