Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device

Hongrang Chen; Haitao Zhang; Yun Shen; Xingliang Dai; Xuanzhi Wang; Kunxue Deng; Xiaoyan Long; Libiao Liu; Xinzhi Zhang; Yongsheng Li; Tao Xu; Tao Xu

doi:10.3389/fbioe.2021.684105

Frontiers in Bioengineering and Biotechnology (Jul 2021)

Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device

Hongrang Chen,
Haitao Zhang,
Yun Shen,
Xingliang Dai,
Xuanzhi Wang,
Kunxue Deng,
Xiaoyan Long,
Libiao Liu,
Xinzhi Zhang,
Yongsheng Li,
Tao Xu,
Tao Xu

Affiliations

Hongrang Chen: Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China
Haitao Zhang: Department of Research & Development, East China Institute of Digital Medical Engineering, Shangrao, China
Yun Shen: Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China
Xingliang Dai: Department of Neurosurgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China
Xuanzhi Wang: Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Kunxue Deng: Department of Research & Development, East China Institute of Digital Medical Engineering, Shangrao, China
Xiaoyan Long: Department of Research & Development, East China Institute of Digital Medical Engineering, Shangrao, China
Libiao Liu: Department of Research & Development, East China Institute of Digital Medical Engineering, Shangrao, China
Xinzhi Zhang: Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing, China
Yongsheng Li: Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China
Tao Xu: Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing, China
Tao Xu: Department of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen, China

DOI: https://doi.org/10.3389/fbioe.2021.684105
Journal volume & issue: Vol. 9

Abstract

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Background: This study aims to design a 3D printed handheld electrospinning device and evaluate its effect on the rapid repair of mouse skin wounds.Methods: The device was developed by Solidworks and printed by Object 350 photosensitive resin printer. The polylactic acid (PLA)/gelatin blend was used as the raw material to fabricate in-situ degradable nanofiber scaffolds. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and water vapor permeability test were used to evaluate the material properties of the scaffolds; cytotoxicity test was performed to evaluate material/residual solvent toxicity, and in situ tissue repair experiments in Balb/c mouse were performed.Results: The 3D printed handheld electrospinning device successfully fabricates PLA/gelatin nanofibrous membrane with uniformly layered nanofibers and good biocompatibility. Animal experiments showed that the mice in the experimental group had complete skin repair.Conclusions: The 3D printed handheld device can achieve in situ repair of full-thickness defects in mouse skin.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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