Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis

Julia TCW; Claudia M.B. Carvalho; Bo Yuan; Shen Gu; Alyssa N. Altheimer; Shane McCarthy; Dheeraj Malhotra; Jonathan Sebat; Arthur J. Siegel; Uwe Rudolph; James R. Lupski; Deborah L. Levy; Kristen J. Brennand

doi:10.1016/j.stemcr.2017.01.010

Stem Cell Reports (Mar 2017)

Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis

Julia TCW,
Claudia M.B. Carvalho,
Bo Yuan,
Shen Gu,
Alyssa N. Altheimer,
Shane McCarthy,
Dheeraj Malhotra,
Jonathan Sebat,
Arthur J. Siegel,
Uwe Rudolph,
James R. Lupski,
Deborah L. Levy,
Kristen J. Brennand

Affiliations

Julia TCW: Departments of Neuroscience, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA
Claudia M.B. Carvalho: Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Bo Yuan: Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Shen Gu: Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Alyssa N. Altheimer: Departments of Neuroscience, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA
Shane McCarthy: Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
Dheeraj Malhotra: F. Hoffmann-La Roche Ltd, 4070 Basel, Switzerland
Jonathan Sebat: Departments of Psychiatry, Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
Arthur J. Siegel: Department of Internal Medicine, McLean Hospital, Belmont, MA 02478, USA
Uwe Rudolph: Laboratory of Genetic Neuropharmacology, McLean Hospital, Belmont, MA 02478, USA
James R. Lupski: Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Deborah L. Levy: Department of Psychiatry, Harvard Medical School, Boston, MA 02115, USA
Kristen J. Brennand: Departments of Neuroscience, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA

DOI: https://doi.org/10.1016/j.stemcr.2017.01.010
Journal volume & issue: Vol. 8, no. 3
pp. 519 – 528

Abstract

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In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.

Published in Stem Cell Reports

ISSN: 2213-6711 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: http://www.cell.com/stem-cell-reports/home

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Abstract

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