Xin yixue (Jun 2022)
Effect and mechanism of IL-37 silencing on osteosarcoma cells
Abstract
Objective To investigate the effect of IL-37 on the proliferation, apoptosis, migration and invasion of osteosarcoma cells and its mechanism. Methods The expression of IL-37 in human normal osteoblasts （hFOB1.19） and osteosarcoma cell lines （U2OS, MG63, Saos-2） was detected by quantitative reverse transcription polymerase chain reaction （RT-qPCR）. The siRNA sequence silencing IL-37 was designed and transfected into the osteosarcoma cell lines. Cells transfected with siRNA sequence silencing IL-37 were assigned into the siIL-37 group, and those transfected with negative controls were allocated into the siNC group. The expression of IL-37 mRNA in osteosarcoma cells after IL-37 silencing was detected. The effect of IL-37 silencing on the proliferation, invasion and migration of osteosarcoma cells was evaluated by CCK-8 and Transwell chamber test. The effect of IL-37 silencing on the apoptosis of osteosarcoma cell lines was detected by flow cytometry. The expression levels of key proteins in the BAX/BCL-2 and Wnt/β-catenin signaling pathway of osteosarcoma cell lines before and after silencing IL-37 were determined western blot. Results The expression levels of IL-37 mRNA in osteosarcoma cell lines U2OS, MG63 and Saos-2 were significantly higher than that in normal osteoblast cell line （all P < 0.05）. For osteosarcoma cell lines U2OS, MG63 and Saos-2, the relative expression levels of IL-37 mRNA in the siIL-37 group were significantly lower than those in the siNC group （all P < 0.05）. Compared with the siNC group, cell proliferation rate was declined, cell apoptosis rate was increased, the quantity of cell migration and invasion was elevated, BAX expression was up-regulated, BCL-2 expression was down-regulated, and the expression levels of c-Myc, β-catenin, TEF1, Cyclin D1 and MMP-7 in the Wnt/β-catenin signal pathway were down-regulated in the siIL-37 group （all P < 0.05）. Conclusions The expression level of IL-37 is up-regulated in osteosarcoma cell lines. Silencing IL-37 can promote the apoptosis of osteosarcoma cells and inhibit the proliferation, migration and invasion. IL-37 plays an important carcinogenic role in the occurrence and development of osteosarcoma possibly through the BAX/BCL-2 and Wnt/ β-catenin signaling pathways.
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