Infection and Drug Resistance (Jul 2022)

MALDI-TOF MS-Based Clustering and Antifungal Susceptibility Tests of Talaromyces marneffei Isolates from Fujian and Guangxi (China)

  • Fang L,
  • Liu M,
  • Huang C,
  • Ma X,
  • Zheng Y,
  • Wu W,
  • Guo J,
  • Huang J,
  • Xu H

Journal volume & issue
Vol. Volume 15
pp. 3449 – 3457

Abstract

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Lili Fang,1– 3,* Minxue Liu,4,* Chunlan Huang,5,* Xiaobo Ma,1– 3 Yanqing Zheng,1– 3 Wenjuan Wu,6 Jian Guo,6,* Jiangshan Huang,1– 3 Heping Xu1– 3 1Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, People’s Republic of China; 2Xiamen Key Laboratory of Genetic Testing, Xiamen, Fujian, People’s Republic of China; 3School of Public Health, Xiamen University, Xiamen, Fujian, People’s Republic of China; 4The Maternal & Child Health Hospital, The Children’s Hospital, The Obstetrics & Gynecology Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, People’s Republic of China; 5Department of Clinical Laboratory, Liuzhou People’s Hospital, Liuzhou, Guangxi, People’s Republic of China; 6Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Heping Xu; Jiangshan, Hung Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, People’s Republic of China, Email [email protected]; [email protected]: Talaromyces marneffei is a life-threatening pathogen that causes systemic talaromycosis in immunocompromised and acquired immunodeficiency syndrome (AIDS) patients. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a tool to cluster T. marneffei isolates is rarely reported and the data on antifungal susceptibility of T. marneffei isolated in the southern region of China, especially in Fujian, is hardly found.Methods: MALDI-TOF MS was used to cluster 135 T. marneffei isolates, and the minimum inhibitory concentration (MIC) values of amphotericin B, itraconazole, posaconazole, voriconazole, fluconazole, anidulafungin, micafungin, caspofungin and 5-fluorocytosine with Sensititre YeastOne™ YO10 assay were measured during January 2017 to October 2020 in Fujian and Guangxi.Results: MALDI-TOF MS correctly identified 100% of the T. marneffei isolates. Hierarchical clustering of MALDI-TOF peak profiles identified four different clusters. MICs for itraconazole, posaconazole, voriconazole and amphotericin B were as follows: ≤ 0.015– 0.03 μg/mL, ≤ 0.008– 0.03 μg/mL, ≤ 0.008– 0.06 μg/mL, ≤ 0.12– 1 μg/mL, respectively. MICs for echinocandins and fluconazole were comparatively high.Conclusion: Since only simple sample preparation is required and since results are available in a short period of time, MALDI-TOF MS can be considered as a method for identification and clustering of T. marneffei. Itraconazole, posaconazole, voriconazole and amphotericin B can be used to treat T. marneffei infected patients due to the low MICs.Keywords: Talaromyces marneffei, MALDI-TOF, antifungal susceptibility, HIV-infected, non-HIV-infected

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