Polymorphisms in WISP1 and NMI Genes and their Effects on the Mortality of Chick Embryos

Abstract

In order to investigate the polymorphisms of two candidate genes, WISP1 (Wnt-1-induced secreted protein) and NMI (N-myc (and STAT) interactor), and their effects on embryonic mortality, 187 dead and 71 normal embryos of AiJiaoHuang (AJH) and 154 dead and 70 normal embryos of ZhuSi (ZS) chicken were collected. Two single nucleotide polymorphisms (SNPs), rs14261761 (T/C) at exon5 of WISP1 and rs14630452 (A/T) at exon5 of NMI, were genotyped by PCR-RFLP (polymerase chain reaction-restriction fragment-length polymorphism). Our results showed that the mortality rates of different genotypes of rs14261761 were significantly different in ZS chicken, and the death risk of TT genotype tended to be higher than that of TC; rs14630452 significantly influenced the embryonic mortality of AJH chicken, and the death risk of TT genotype was 4 and 2 times higher than that of AA and AT genotypes, respectively (TT>AT>AA). These results indicated that these two SNPs both affected the chick embryonic mortality.

Keywords

• chicken
• embryonic mortality
• nmi
• snp
• wisp1