Virology Journal (Aug 2009)

The complete genomic sequence of an in vivo low replicating BLV strain

  • Ceriani Carolina,
  • Gutierrez Silvina,
  • Dolcini Guillermina,
  • Dube Dipak K,
  • Abbott Lynn,
  • Dube Syamalima,
  • Juliarena Marcela,
  • Ferrer Jorge,
  • Perzova Raisa,
  • Poiesz Bernard J

DOI
https://doi.org/10.1186/1743-422X-6-120
Journal volume & issue
Vol. 6, no. 1
p. 120

Abstract

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Abstract DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate, low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV ARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequences including an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina. Characterization of BLV ARG 41's deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed.