Galectin-related protein from Oreochromis niloticus: Molecular, and transcriptional features and biological responses against microbial pathogens

Guoling Luo; Jinzhong Niu; Junfeng Fang; Jufen Tang; Yishan Lu; Yu Huang; Jichang Jian

Aquaculture Reports (Nov 2021)

Galectin-related protein from Oreochromis niloticus: Molecular, and transcriptional features and biological responses against microbial pathogens

Guoling Luo,
Jinzhong Niu,
Junfeng Fang,
Jufen Tang,
Yishan Lu,
Yu Huang,
Jichang Jian

Affiliations

Guoling Luo: Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China
Jinzhong Niu: Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China
Junfeng Fang: Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China
Jufen Tang: Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China
Yishan Lu: Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China
Yu Huang: Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China; Corresponding authors at: College of Fishery, Guangdong Ocean University, Zhanjiang, Guangdong, China.
Jichang Jian: Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China; Corresponding authors at: College of Fishery, Guangdong Ocean University, Zhanjiang, Guangdong, China.

Journal volume & issue: Vol. 21
p. 100782

Abstract

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Galectin is a kind of lectin that can promote the cell adhesion, proliferation, and phagocytosis and participates in immune regulation and destruction of microbes. In this study, a galectin-related protein (On-GRP) homolog was identified from Nile tilapia, and its expression level and antibacterial ability were studied. The Open Reading Frame, which encoded a 179 amino acid protein (GenBank accession no: XM_005733705.1), was 537 bp. The On-GRP possessed a carbohydrate recognition domain. qRT-PCR showed that the On-GRP was detected in the examined tissues in healthy Nile tilapia, and the highest expression levels was observed in the skin. After challenge with Streptococcus agalactiae, the On-GRP expression was up-regulated significantly (P < 0.05) in the whole blood, head kidney, gills and thymus. In addition, the recombinant On-GRP (rOn-GRP) could agglutinate and bind Streptococcus agalactiae and Aeromonas hydrophila. In vitro and in vivo bacteriostatic tests showed that the rOn-GRP could inhibit the growth of bacteria. Besides, the rOn-GRP could promote the phagocytic ability of macrophages to engulf bacteria. Over all, this study indicated On-GRP is involved in the immune responses of Nile tilapia against bacterial pathogens.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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