Evaluation of the Free Radical Scavenging Activities of Ellagic Acid and Ellagic Acid Peracetate by EPR Spectrometry

Ajit Kumar; Preeti Kaushik; Sandra Incerpi; Jens Z. Pedersen; Sanjay Goel; Ashok K. Prasad; Vishwajeet Rohil; Virinder S. Parmar; Luciano Saso; Christophe Len

doi:10.3390/molecules26164800

Molecules (Aug 2021)

Evaluation of the Free Radical Scavenging Activities of Ellagic Acid and Ellagic Acid Peracetate by EPR Spectrometry

Ajit Kumar,
Preeti Kaushik,
Sandra Incerpi,
Jens Z. Pedersen,
Sanjay Goel,
Ashok K. Prasad,
Vishwajeet Rohil,
Virinder S. Parmar,
Luciano Saso,
Christophe Len

Affiliations

Ajit Kumar: Department of Chemistry, SRM University, Delhi-NCR, Haryana, 39, RGEC, Sonepat 131 029, India
Preeti Kaushik: Department of Chemistry, SRM University, Delhi-NCR, Haryana, 39, RGEC, Sonepat 131 029, India
Sandra Incerpi: Department of Sciences, University of Rome “Roma Tre”, 00146 Rome, Italy
Jens Z. Pedersen: Department of Biology, University of Rome “Tor Vergata”, Via della Ricerca, Scientifica 1, 00133 Rome, Italy
Sanjay Goel: Department of Biochemistry, V. P. Chest Institute, University of Delhi, Delhi 110 007, India
Ashok K. Prasad: Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi 110 007, India
Vishwajeet Rohil: Department of Biochemistry, V. P. Chest Institute, University of Delhi, Delhi 110 007, India
Virinder S. Parmar: Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi 110 007, India
Luciano Saso: Department of Physiology and Pharmacology “Vittorio Erspamer”, Sapienza University, P. le. Aldo Moro 5, 00185 Rome, Italy
Christophe Len: Institute of Chemistry for Life and Health Sciences, Chimie ParisTech, PSL Research University, CNRS, UMR8060, 11 rue Pierre et Marie Curie, F-75005 Paris, France

DOI: https://doi.org/10.3390/molecules26164800
Journal volume & issue: Vol. 26, no. 16
p. 4800

Abstract

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The purpose of this study was to examine the free radical scavenging and antioxidant activities of ellagic acid (EA) and ellagic acid peracetate (EAPA) by measuring their reactions with the radicals, 2,2-diphenyl-1-picrylhydrazyl and galvinoxyl using EPR spectroscopy. We have also evaluated the influence of EA and EAPA on the ROS production in L-6 myoblasts and rat liver microsomal lipid peroxidation catalyzed by NADPH. The results obtained clearly indicated that EA has tremendous ability to scavenge free radicals, even at concentration of 1 µM. Interestingly even in the absence of esterase, EAPA, the acetylated product of EA, was also found to be a good scavenger but at a relatively slower rate. Kinetic studies revealed that both EA and EAPA have ability to scavenge free radicals at the concentrations of 1 µM over extended periods of time. In cellular systems, EA and EAPA were found to have similar potentials for the inhibition of ROS production in L-6 myoblasts and NADPH-dependent catalyzed microsomal lipid peroxidation.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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