Generation of mixed murine organoids to model cellular interactions

Ana Krotenberg Garcia; Jacco van Rheenen; Saskia Jacoba Elisabeth Suijkerbuijk

STAR Protocols (Dec 2021)

Generation of mixed murine organoids to model cellular interactions

Ana Krotenberg Garcia,
Jacco van Rheenen,
Saskia Jacoba Elisabeth Suijkerbuijk

Affiliations

Ana Krotenberg Garcia: Department of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands; Corresponding author
Jacco van Rheenen: Department of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands
Saskia Jacoba Elisabeth Suijkerbuijk: Department of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 100997

Abstract

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Summary: Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis.For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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