The structure of Chlamydomonas LOV1 as revealed by time-resolved serial synchrotron crystallography

Abstract

The photo-reaction of the LOV1 domain of the Chlamydomonas reinhardtii phototropin is investigated by room-temperature time-resolved serial crystallography. A covalent adduct forms between the C4a atom of the central flavin-mononucleotide chromophore and a protein cysteine. The structure of the adduct is very similar to that of LOV2 determined 23 years ago from the maidenhair fern Phy3.

Keywords

• time-resolved serial synchrotron crystallography
• tr-ssx
• room-temperature crystallography
• blue-light photoreceptors
• chlamydomonas reinhardtii
• crphotlov1
• structural dynamics
• light–oxygen–voltage domains