Propagation of gnetum by tissue culture technique

Abstract

Plantlet regeneration from tissue culture of gnetum could be induced using two pathways depending upon explant types. Leaf lamina yielded somatic embryos through somatic embryogenesis in basal MS supplemented with the same concentration of BA and TDZ at 0.5 mg/l. Using cluster of flowers or ovules in the same medium formula, however, callus formation took place and developed into meristemoid structures. These calli are called meristematic nodular calli and the pathway was organogenesis. Both pathways of plantlet regeneration could be applied for mass propagation of gnetum on a commercial scale in the future.

Keywords

• propagation of gnetum
• tissue culture technique
• gnetum