BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage

Zhihua Kang; Pan Fu; Allen L. Alcivar; Haiqing Fu; Christophe Redon; Tzeh Keong Foo; Yamei Zuo; Caiyong Ye; Ryan Baxley; Advaitha Madireddy; Remi Buisson; Anja-Katrin Bielinsky; Lee Zou; Zhiyuan Shen; Mirit I. Aladjem; Bing Xia

doi:10.1038/s41467-021-26227-6

Nature Communications (Oct 2021)

BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage

Zhihua Kang,
Pan Fu,
Allen L. Alcivar,
Haiqing Fu,
Christophe Redon,
Tzeh Keong Foo,
Yamei Zuo,
Caiyong Ye,
Ryan Baxley,
Advaitha Madireddy,
Remi Buisson,
Anja-Katrin Bielinsky,
Lee Zou,
Zhiyuan Shen,
Mirit I. Aladjem,
Bing Xia

Affiliations

Zhihua Kang: Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey
Pan Fu: Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey
Allen L. Alcivar: Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey
Haiqing Fu: Developmental Therapeutics Group, Center for Cancer Research, National Cancer Institute
Christophe Redon: Developmental Therapeutics Group, Center for Cancer Research, National Cancer Institute
Tzeh Keong Foo: Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey
Yamei Zuo: Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey
Caiyong Ye: Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey
Ryan Baxley: Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota
Advaitha Madireddy: Department of Pediatric Hematology/Oncology, Rutgers Cancer Institute of New Jersey
Remi Buisson: Massachusetts General Hospital Cancer Center and Harvard Medical School
Anja-Katrin Bielinsky: Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota
Lee Zou: Massachusetts General Hospital Cancer Center and Harvard Medical School
Zhiyuan Shen: Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey
Mirit I. Aladjem: Developmental Therapeutics Group, Center for Cancer Research, National Cancer Institute
Bing Xia: Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey

DOI: https://doi.org/10.1038/s41467-021-26227-6
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

Read online

Tumor suppressor BRCA2 is known to stabilize and restart stalled DNA replication forks. Here the authors show that BRCA2 is recruited to the replication fork through its interaction with MCM10 and inhibits Primase-Polymerase-mediated repriming, lesion bypass and single strand DNA gap formation after DNA damage.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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