Protocol to detect antigen-specific nasal-resident T cells in humans

Abstract

Summary: Specialized T cells are located in the nasal cavity and act as the first line of defense against respiratory viral infection. Here, we present a protocol for the detection and characterization of antigen-specific nasal-resident T cells. We detail steps for localized nasal swabbing to collect the nasal samples. We then describe IFN-γ ELISpot and an activation-induced marker assay to detect and characterize antigen-specific nasal-resident T cells.For complete details on the use and execution of this protocol, please refer to Lim et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell isolation
• Cell-based Assays
• Health Sciences
• Clinical Protocol
• Immunology
• Microbiology