Combined transcriptome and metabolome analysis provides insight into the ERF073 – Malic acid network in pakchoi under submergence stress
Dan-dan Xi,
Lu Gao,
Li-ao Ge,
Hong-fang Zhu,
Li-ming Miao,
Ding-yu Zhang,
Chang-wei Zhang,
Ying Li,
Yan-xiao Dong,
Xi-lin Hou,
Yu-ying Zhu,
Zhao-hui Zhang,
Xiao-feng Li
Affiliations
Dan-dan Xi
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; College of Food Science and Technology, Zhejiang Pharmaceutical University, 315599, China
Lu Gao
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Li-ao Ge
Jinshan Agricultural Technology Extension Center, Shanghai 201599, China
Hong-fang Zhu
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Li-ming Miao
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Ding-yu Zhang
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Chang-wei Zhang
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Ying Li
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Yan-xiao Dong
Shanghai Agricultural Science and Technology Service Center, Shanghai 200335, China
Xi-lin Hou
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Yu-ying Zhu
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Zhao-hui Zhang
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; Corresponding authors.
Xiao-feng Li
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; Corresponding authors.
Pakchoi is sensitive to oxygen deficiency caused by submergence; however, the molecular mechanisms underlying its response to these conditions remains unclear. To determine these mechanisms, two pakchoi cultivars, Heiyoudong (HYD) and Suzhouqing (SZQ), were subjected to submergence for 1 h and 5 h, respectively. Following this treatment, the plants were transferred back to the control conditions for recovery. HYD exhibited higher survival rate than SZQ after recovery. Leaves from 1 h stress, 5 h stress, and subsequent 5-h recovery phases were harvested for metabolome and transcriptome analyses. Data analysis revealed that 206 differentially accumulated metabolites (DAMs) were identified between SZQ and HYD after 1 h stress (S1 vs H1) and 330 DAMs were identified between SZQ and HYD after 5 h stress (S5 vs H5) at stress stage. During recovery stage, 124 and 310 DAMs were found in S1 vs H1 and S5 vs H5, respectively. Additionally, 553 commonly differentially expressed genes (DEGs) were found both in S1 vs H1 and S5 vs H5 at the stress stage, while 458 DEGs were commonly found in the two comparison groups at the recovery stage. Among these DEGs, ERF073 was down-regulated, as further confirmed by qRT-PCR. KEGG pathway analysis revealed that DAMs and DEGs were mainly enriched in metabolic pathways. Taken together, these findings indicate that ERF073 may regulate malic acid accumulation in pakchoi, increasing its resistance to submergence.
