Bovine Parapoxvirus: Isolation and pathogenicity studies

Abstract

A disease characterized by papules, nodules, vesicles, pustules and ulcers on teats and udder as well as drastic drop in milk production was seen among a cattle farm in Fayoum Governorate, Egypt. A virus was isolated by inoculation of vesicle and scrap homogenate pool from infected cattle into the chorioallantoic membrane of specific pathogen free embryonated chicken eggs. The virus was identified by presence of pock lesions, intracytoplasmic inclusion bodies on the chorioallantoic membrane, polymerase chain reaction and immunohistochemistry of the inoculated membrane. A novel pathogenicity model was developed via ear pinna inoculation of Swiss mice. The virus produced vesicular and ulcerative lesions at the site of inoculation in inoculated mice. The virus identity was confirmed by the presence of intracytoplasmic viral antigens by immunohistochemistry

Keywords

• bovine; parapoxvirus; isolation; pathogenicity; studies