CHANGES IN PROOXIDANT, THYROID AND IODINE STATUS IN HYPOTHYROID RATS UNDER THE ACTION OF ALCOHOL TINCTURE FROM LEMNA MINOR FROND

Abstract

In hypothyroidism, there are changes associated with a lack of thyroid hormones, which affect antioxidant activity by regulating metabolic processes, antioxidant content and lipid peroxidation (LPO). The lack of thyroid hormones leads to significant metabolic disorders such as the intensification of oxidative stress. It can be assumed that the degree of disruption of physiological processes in hypothyroidism will largely depend on the activity of the LPO reactions and the reserve of antioxidant protection. Therefore, it becomes evident that the effective treatment of hypothyroidism requires the study of new drugs that can normalize the violation of prooxidant-antioxidant balance and changes in thyroid and iodine status in the body. The aim. Evaluation of the effectiveness of 30% tincture from Lemna minor frond on prooxidant potential, thyroid and iodine status in serum and tissues of rats with mercazolyl-induced hypothyroidism. Research objects and methods. The object of the study was 30% tincture from Lemna minor frond standardized in accordance with the requirements of the State Pharmacopoeia of Ukraine and the European Pharmacopoeia. Experimental hypothyroidism in rats was induced by daily administration of 0.05% aqueous solution of mercazolyl instead of drinking water for 30 days. The studied tincture of the animal were received intragastrically on the 21st day, starting from the 13th day of administration of mercazolyl. Lipid hydroperoxides content, aconitase activity and thyroid hormone concentration was determined in the serum, the content of lipid hydroperoxides was determined in the mitochondrial and postmitochondrial fractions of the liver, the content of total iodine – In the thyroid gland. Results. Experimental hypothyroidism is characterized by changes in prooxidant, thyroid and iodine status in rats, which was manifested by a statistically significant decrease in lipid hydroperoxides content in the mitochondrial and postmitochondrial fractions of the liver, aconitase activity and thyroid hormones concentration in blood serum and ioine content in the thyroid gland. The use of 30% tincture from Lemna minor frond helped to restore prooxidant, thyroid and iodine status, as evidenced by the restoration of lipid hydroperoxides levels, aconitase activity, total and free thyroxine and triiodothyronine concentration, and iodine content in thyroid tissue to the level of intact control. Conclusions. The use of 30% tincture from Lemna minor frond restored prooxidant, thyroid and iodine status in hypothyroid rats, as evidenced by an increase the total and free thyroxine and triiodothyronine in serum, lipid hydroperoxides, thyroid iodine and aconitase activity

Keywords

• hypothyroidism
• alcohol tincture from lemna minor frond
• prooxidant status
• lipid hydroperoxide
• aconitase
• thyroid hormones.