Journal of Extracellular Biology (Oct 2023)
Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters
- Faezeh Shekari,
- Faisal J. Alibhai,
- Hossein Baharvand,
- Verena Börger,
- Stefania Bruno,
- Owen Davies,
- Bernd Giebel,
- Mario Gimona,
- Ghasem Hosseini Salekdeh,
- Lorena Martin‐Jaular,
- Suresh Mathivanan,
- Inge Nelissen,
- Esther Nolte‐’t Hoen,
- Lorraine O'Driscoll,
- Francesca Perut,
- Stefano Pluchino,
- Gabriella Pocsfalvi,
- Carlos Salomon,
- Carolina Soekmadji,
- Simon Staubach,
- Ana Claudia Torrecilhas,
- Ganesh Vilas Shelke,
- Tobias Tertel,
- Dandan Zhu,
- Clotilde Théry,
- Kenneth Witwer,
- Rienk Nieuwland
Affiliations
- Faezeh Shekari
- Department of Stem Cells and Developmental Biology, Cell Science Research Center Royan Institute for Stem Cell Biology and Technology, ACECR Tehran Iran
- Faisal J. Alibhai
- University Health Network Toronto Canada
- Hossein Baharvand
- Department of Stem Cells and Developmental Biology, Cell Science Research Center Royan Institute for Stem Cell Biology and Technology, ACECR Tehran Iran
- Verena Börger
- Institute for Transfusion Medicine University Hospital Essen, University of Duisburg‐Essen Essen Germany
- Stefania Bruno
- Department of Medical Sciences and Molecular Biotechnology Center University of Torino Turin Italy
- Owen Davies
- School of Sport, Exercise and Health SciencesLoughborough University Loughborough UK
- Bernd Giebel
- Institute for Transfusion Medicine University Hospital Essen, University of Duisburg‐Essen Essen Germany
- Mario Gimona
- GMP Unit Spinal Cord Injury & Tissue Regeneration Centre Salzburg (SCI‐TReCS) and Research Program “Nanovesicular Therapies” Paracelsus Medical University Salzburg Austria
- Ghasem Hosseini Salekdeh
- Faculty of Natural Sciences Macquarie University North Ryde NSW Australia
- Lorena Martin‐Jaular
- Institut Curie, INSERM U932 and Curie CoreTech Extracellular Vesicles PSL Research University Paris France
- Suresh Mathivanan
- Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science La Trobe University Melbourne VIC Australia
- Inge Nelissen
- VITO (Flemish Institute for Technological Research), Health department Boeretang Belgium
- Esther Nolte‐’t Hoen
- Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands
- Lorraine O'Driscoll
- School of Pharmacy and Pharmaceutical Sciences & Trinity Biomedical Sciences Institute Trinity College Dublin Dublin Ireland
- Francesca Perut
- Biomedical Science and Technologies and Nanobiotechnology Lab IRCCS Istituto Ortopedico Rizzoli Bologna Italy
- Stefano Pluchino
- Department of Clinical Neurosciences University of Cambridge Cambridge UK
- Gabriella Pocsfalvi
- Institute of Biosciences and BioResources National Research Council Naples Italy
- Carlos Salomon
- Translational Extracellular Vesicles in Obstetrics and Gynae‐Oncology Group, UQ Centre for Clinical Research, Royal Brisbane and Women's Hospital, Faculty of Medicine The University of Queensland Brisbane Australia
- Carolina Soekmadji
- School of Biomedical Sciences, Faculty of Medicine University of Queensland Brisbane Australia
- Simon Staubach
- Sartorius – BIA Separations Slovenia
- Ana Claudia Torrecilhas
- Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários, Departamento de Ciências Farmacêuticas Universidade Federal de São Paulo (UNIFESP) SP Brazil
- Ganesh Vilas Shelke
- Neurosciences and Cellular and Structural Biology Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health Bethesda Maryland USA
- Tobias Tertel
- Institute for Transfusion Medicine University Hospital Essen, University of Duisburg‐Essen Essen Germany
- Dandan Zhu
- The Ritchie Centre Hudson Institute of Medical Research Clayton VIC Australia
- Clotilde Théry
- Institut Curie, INSERM U932 and Curie CoreTech Extracellular Vesicles PSL Research University Paris France
- Kenneth Witwer
- Departments of Molecular and Comparative Pathobiology and Neurology and Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease Johns Hopkins University Baltimore Maryland USA
- Rienk Nieuwland
- Laboratory of Experimental Clinical Chemistry, Department of Clinical Chemistry, Amsterdam University Medical Centers Location AMC, University of Amsterdam Amsterdam The Netherlands
- DOI
- https://doi.org/10.1002/jex2.115
- Journal volume & issue
-
Vol. 2,
no. 10
pp. n/a – n/a
Abstract
Abstract Cell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM‐derived EVs (CCM‐EV). The CCM‐EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell‐specific recommendations may need to be established for non‐mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM‐EV research.
Keywords
