Anti-proliferative activity on drug-sensitive and drug-resistant erythroleukemic cells of Samor Pipek (Terminalia bellirica (Gaertn.) Roxb.) fruit extracts

Abstract

Introduction: Cancer is a major cause of death in Thailand. Drug resistance in cancer is a significant obstacle to the success of chemotherapy in cancer. There are several researches indicate the potential of herb in the inhibition of cancer cell proliferation for applying in cancer treatment. Objectives: To study anti-proliferative activity of Samor Pipek (Terminalia bellirica (Gaertn.) Roxb.) fruit extracts against drug-sensitive and drug-resistant erythroleukemic cells. Materials and methods: Samor Pipek fruits were extracted with acetonitrile, ethanol, and water. Anti-proliferative activity on drug-sensitive and drug-resistant erythroleukemic cells were determined by flow cytometer. Results: Concentration of acetonitrile, ethanol and water extracts for inhibiting drug-sensitive erythroleukemic cell proliferation by 50% (IC50) were 24.70±8.05 μg/mL, 24.02±2.51 μg/mL and 13.09±3.40 μg/mL, respectively. IC50 of acetonitrile, ethanol and water extracts to inhibit drug- resistant erythroleukemic cell proliferation were 13.95±2.50 μg/mL, 20.45±10.10 μg/mL and 27.39±7.16 μg/mL, respectively. The resistance values of acetonitrile, ethanol and water extracts were 0.56, 0.85 and 2.09, respectively. Conclusion: Acetonitrile, ethanol and water extracts could inhibit drug-sensitive and drug-resistant erythroleukemic cell proliferation in μg/mL range. Journal of Associated Medical Sciences 2017; 50(1): 64-70. Doi: 10.14456/jams.2017.6

Keywords

• Samor Pipek
• erythroleukimic cell
• K562
• drug resistance.