Data on recurrent somatic embryogenesis and in vitro micropropagation of Cnidium officinale Makino

Data in Brief. 2018;19:2311-2314

 

Journal Homepage

Journal Title: Data in Brief

ISSN: 2352-3409 (Online)

Publisher: Elsevier

LCC Subject Category: Medicine: Medicine (General): Computer applications to medicine. Medical informatics | Science: Science (General)

Country of publisher: United States

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS

Muhammad Adil (Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea)
Dong Il Kang (Department of Horticulture, Division of Applied Life Science Graduate School (BK 21 Plus Program), Gyeongsang National University, Jinju 52828, Republic of Korea)
Byoung Ryong Jeong (Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea; Department of Horticulture, Division of Applied Life Science Graduate School (BK 21 Plus Program), Gyeongsang National University, Jinju 52828, Republic of Korea; Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea; Corresponding author at: Department of Horticulture, Division of Applied Life Science, Graduate School (BK 21 Plus Program), Gyeongsang National University, Jinju 52828, Republic of Korea.)

EDITORIAL INFORMATION

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Time From Submission to Publication: 4 weeks

 

Abstract | Full Text

Cnidium officinale Makino, a perennial herb of the family Umbelliferae, is a well-known medicinal plant in oriental medicine with antidiabetic, tumor metastatic, antiplatelet, antimicrobial and insecticidal properties. Hence, C. officinale does not produce seed the plant tissue culture is the viable alternative for its propagation. Node explant from in vitro grown C. officinale Makino was cultured on MS medium supplemented with plant growth regulators (PGRs) like 2,4-Dichlorophenoxyacetic acid (2,4-D) or/and 6-Benzylaminopurine (BA). It was aimed to investigate the optimal concentration and combination of 2,4-D and BA for somatic embryogenesis in node explant of C. officinale Makino. The embryogenic callus was induced on node explant after four weeks in MS medium containing 1.5 mg L−1 2,4-D and 0.5 mg L−1 BA. The translucent white, embryogenic callus was subcultured on the respective medium and individual well-structured somatic embryos were observed. Heart and cotyledon stage embryos were pictured under a stereomicroscope. The individual somatic embryos (SE) were transferred to MS medium without PGRs (MS0) and 100% germination was observed. Repeated subculturing of the embryogenic callus for five months resulted in recurrent somatic embryogenesis but with a gradual decline in number.