Nature Communications (May 2021)
Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding
- Klaas Yperman,
- Anna C. Papageorgiou,
- Romain Merceron,
- Steven De Munck,
- Yehudi Bloch,
- Dominique Eeckhout,
- Qihang Jiang,
- Pieter Tack,
- Rosa Grigoryan,
- Thomas Evangelidis,
- Jelle Van Leene,
- Laszlo Vincze,
- Peter Vandenabeele,
- Frank Vanhaecke,
- Martin Potocký,
- Geert De Jaeger,
- Savvas N. Savvides,
- Konstantinos Tripsianes,
- Roman Pleskot,
- Daniel Van Damme
Affiliations
- Klaas Yperman
- Department of Plant Biotechnology and Bioinformatics, Ghent University
- Anna C. Papageorgiou
- CEITEC-Central European Institute of Technology, Masaryk University
- Romain Merceron
- Department of Biochemistry and Microbiology, Ghent University
- Steven De Munck
- Department of Biochemistry and Microbiology, Ghent University
- Yehudi Bloch
- Department of Biochemistry and Microbiology, Ghent University
- Dominique Eeckhout
- Department of Plant Biotechnology and Bioinformatics, Ghent University
- Qihang Jiang
- Department of Plant Biotechnology and Bioinformatics, Ghent University
- Pieter Tack
- Department of Chemistry, X-ray Microspectroscopy and Imaging – XMI Research Unit, Ghent University
- Rosa Grigoryan
- Department of Chemistry, Atomic & Mass Spectrometry – A&MS Research Unit, Ghent University
- Thomas Evangelidis
- CEITEC-Central European Institute of Technology, Masaryk University
- Jelle Van Leene
- Department of Plant Biotechnology and Bioinformatics, Ghent University
- Laszlo Vincze
- Department of Chemistry, X-ray Microspectroscopy and Imaging – XMI Research Unit, Ghent University
- Peter Vandenabeele
- Department of Chemistry, X-ray Microspectroscopy and Imaging – XMI Research Unit, Ghent University
- Frank Vanhaecke
- Department of Chemistry, Atomic & Mass Spectrometry – A&MS Research Unit, Ghent University
- Martin Potocký
- Institute of Experimental Botany, Academy of Sciences of the Czech Republic
- Geert De Jaeger
- Department of Plant Biotechnology and Bioinformatics, Ghent University
- Savvas N. Savvides
- Department of Biochemistry and Microbiology, Ghent University
- Konstantinos Tripsianes
- CEITEC-Central European Institute of Technology, Masaryk University
- Roman Pleskot
- Department of Plant Biotechnology and Bioinformatics, Ghent University
- Daniel Van Damme
- Department of Plant Biotechnology and Bioinformatics, Ghent University
- DOI
- https://doi.org/10.1038/s41467-021-23314-6
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 11
Abstract
AtEH/Pan1 proteins contain two N-terminal Eps15 homology (EH) domains and are subunits of the endocytic TPLATE complex present in plants. Here, the authors combine X-ray crystallography, NMR and MD simulations with biochemical and in planta analysis to characterize the two AtEH1/Pan1 EH domains and reveal their structural differences and complementary functional roles.
