Bioinformatics and Biology Insights (May 2024)

DENV-2 Outbreak Associated With Cosmopolitan Genotype Emergence in Western Brazilian Amazon

  • Jessiane Rodrigues Ribeiro,
  • Tárcio Peixoto Roca,
  • Gleense dos Santos Cartonilho,
  • Ana Maísa Passos-Silva,
  • Hillquias Monteiro Moreira,
  • Karolaine Santos Teixeira,
  • André Luiz Ferreira da Silva,
  • Celina Aparecida Bertoni Lugtenburg,
  • Alcione Oliveira dos Santos,
  • Juan Miguel Villalobos Salcedo,
  • Valdinete Alves do Nascimento,
  • Victor Costa de Souza,
  • Rosemary Aparecida Roque,
  • Marco Aurélio Krieger,
  • Felipe Gomes Naveca,
  • Rita de Cássia Pontello Rampazzo,
  • Deusilene Souza Vieira

DOI
https://doi.org/10.1177/11779322241251581
Journal volume & issue
Vol. 18

Abstract

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Background: Dengue virus (DENV) causes an important disease and directly affects public health, being the arbovirus that presents the highest number of infections and deaths in the Western Brazilian Amazon. This virus is divided into 4 serotypes that have already circulated in the region. Methodology: Molecular characterization of a cohort containing 841 samples collected from febrile patients between 2021 and 2023 was analyzed using a commercial kit to detect the main arboviruses circulating in Brazil: Zika, DENV-1, DENV-2, DENV-3, DENV-4 and, Chikungunya. Subsequently, Sanger sequencing was performed for positive samples. Results: The cohort detected 162 positive samples, 12 for DENV-1 and 150 identified as DENV-2, indicating co-circulation of serotypes. The samples were subjected to sequencing and the analysis of the sequences that obtained good quality revealed that 5 samples belonged to the V genotype of DENV-1 and 46 were characterized as DENV-2 Cosmopolitan genotype—lineage 5. Conclusion: The results allowed us to identify for the first time the Cosmopolitan genotype in Rondônia, Brazilian Western Amazon, and its fast spread dispersion.