Light-Emitting Diode-Based Illumination System for In Vitro Photodynamic Therapy

Defu Chen; Huifen Zheng; Zhiyong Huang; Huiyun Lin; Zhidong Ke; Shusen Xie; Buhong Li

doi:10.1155/2012/920671

International Journal of Photoenergy (Jan 2012)

Light-Emitting Diode-Based Illumination System for In Vitro Photodynamic Therapy

Defu Chen,
Huifen Zheng,
Zhiyong Huang,
Huiyun Lin,
Zhidong Ke,
Shusen Xie,
Buhong Li

Affiliations

Defu Chen: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fujian 350007, China
Huifen Zheng: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fujian 350007, China
Zhiyong Huang: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fujian 350007, China
Huiyun Lin: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fujian 350007, China
Zhidong Ke: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fujian 350007, China
Shusen Xie: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fujian 350007, China
Buhong Li: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fujian 350007, China

DOI: https://doi.org/10.1155/2012/920671
Journal volume & issue: Vol. 2012

Abstract

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The aim of this study is to develop a light-emitting diode- (LED-) based illumination system that can be used as an alternative light source for in vitro photodynamic therapy (PDT). This illumination system includes a red LED array composed of 70 LEDs centered at 643 nm, an air-cooling unit, and a specific-designed case. The irradiance as a function of the irradiation distance between the LED array and the sample, the homogeneity and stability of irradiation, and the effect of long-time irradiation on culture medium temperature were characterized. Furthermore, the survival rate of the CNE1 cells that sensitized with 5-aminolevulinic acid after PDT treatment was evaluated to demonstrate the efficiency of the new LED-based illumination system. The obtained results show that the LED-based illumination system is a promising light source for in vitro PDT that performed in standard multiwell plate.

Published in International Journal of Photoenergy

ISSN: 1110-662X (Print); 1687-529X (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Technology: Mechanical engineering and machinery: Renewable energy sources
Website: https://www.hindawi.com/journals/ijp/

About the journal