Communications Chemistry (May 2023)
Mirror proteorhodopsins
- Ivan S. Okhrimenko,
- Kirill Kovalev,
- Lada E. Petrovskaya,
- Nikolay S. Ilyinsky,
- Alexey A. Alekseev,
- Egor Marin,
- Tatyana I. Rokitskaya,
- Yuri N. Antonenko,
- Sergey A. Siletsky,
- Petr A. Popov,
- Yuliya A. Zagryadskaya,
- Dmytro V. Soloviov,
- Igor V. Chizhov,
- Dmitrii V. Zabelskii,
- Yury L. Ryzhykau,
- Alexey V. Vlasov,
- Alexander I. Kuklin,
- Andrey O. Bogorodskiy,
- Anatolii E. Mikhailov,
- Daniil V. Sidorov,
- Siarhei Bukhalovich,
- Fedor Tsybrov,
- Sergey Bukhdruker,
- Anastasiia D. Vlasova,
- Valentin I. Borshchevskiy,
- Dmitry A. Dolgikh,
- Mikhail P. Kirpichnikov,
- Ernst Bamberg,
- Valentin I. Gordeliy
Affiliations
- Ivan S. Okhrimenko
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Kirill Kovalev
- European Molecular Biology Laboratory
- Lada E. Petrovskaya
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS
- Nikolay S. Ilyinsky
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Alexey A. Alekseev
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Egor Marin
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Tatyana I. Rokitskaya
- Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
- Yuri N. Antonenko
- Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
- Sergey A. Siletsky
- Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
- Petr A. Popov
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Yuliya A. Zagryadskaya
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Dmytro V. Soloviov
- European Molecular Biology Laboratory
- Igor V. Chizhov
- Institute for Biophysical Chemistry, Hannover Medical School
- Dmitrii V. Zabelskii
- European XFEL
- Yury L. Ryzhykau
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Alexey V. Vlasov
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Alexander I. Kuklin
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Andrey O. Bogorodskiy
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Anatolii E. Mikhailov
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Daniil V. Sidorov
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Siarhei Bukhalovich
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Fedor Tsybrov
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Sergey Bukhdruker
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Anastasiia D. Vlasova
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Valentin I. Borshchevskiy
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Dmitry A. Dolgikh
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS
- Mikhail P. Kirpichnikov
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS
- Ernst Bamberg
- Max Planck Institute of Biophysics
- Valentin I. Gordeliy
- Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CNRS, CEA
- DOI
- https://doi.org/10.1038/s42004-023-00884-8
- Journal volume & issue
-
Vol. 6,
no. 1
pp. 1 – 16
Abstract
Abstract Proteorhodopsins (PRs), bacterial light-driven outward proton pumps comprise the first discovered and largest family of rhodopsins, they play a significant role in life on the Earth. A big remaining mystery was that up-to-date there was no described bacterial rhodopsins pumping protons at acidic pH despite the fact that bacteria live in different pH environment. Here we describe conceptually new bacterial rhodopsins which are operating as outward proton pumps at acidic pH. A comprehensive function-structure study of a representative of a new clade of proton pumping rhodopsins which we name “mirror proteorhodopsins”, from Sphingomonas paucimobilis (SpaR) shows cavity/gate architecture of the proton translocation pathway rather resembling channelrhodopsins than the known rhodopsin proton pumps. Another unique property of mirror proteorhodopsins is that proton pumping is inhibited by a millimolar concentration of zinc. We also show that mirror proteorhodopsins are extensively represented in opportunistic multidrug resistant human pathogens, plant growth-promoting and zinc solubilizing bacteria. They may be of optogenetic interest.
