NopP2 effector of Bradyrhizobium elkanii USDA61 is a determinant of nodulation in Vigna radiata cultivars

Pongdet Piromyou; Natcha Pruksametanan; Hien P. Nguyen; Pongpan Songwattana; Jenjira Wongdee; Phongkeat Nareephot; Teerana Greetatorn; Kamonluck Teamtisong; Panlada Tittabutr; Nantakorn Boonkerd; Shusei Sato; Pakpoom Boonchuen; Shin Okazaki; Neung Teaumroong

doi:10.1038/s41598-024-75294-4

Scientific Reports (Oct 2024)

NopP2 effector of Bradyrhizobium elkanii USDA61 is a determinant of nodulation in Vigna radiata cultivars

Pongdet Piromyou,
Natcha Pruksametanan,
Hien P. Nguyen,
Pongpan Songwattana,
Jenjira Wongdee,
Phongkeat Nareephot,
Teerana Greetatorn,
Kamonluck Teamtisong,
Panlada Tittabutr,
Nantakorn Boonkerd,
Shusei Sato,
Pakpoom Boonchuen,
Shin Okazaki,
Neung Teaumroong

Affiliations

Pongdet Piromyou: Institute of Research and Development, Suranaree University of Technology
Natcha Pruksametanan: School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Hien P. Nguyen: Institute of Global Innovation Research (IGIR), Tokyo University of Agriculture and Technology (TUAT)
Pongpan Songwattana: Institute of Research and Development, Suranaree University of Technology
Jenjira Wongdee: Institute of Research and Development, Suranaree University of Technology
Phongkeat Nareephot: School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Teerana Greetatorn: Institute of Research and Development, Suranaree University of Technology
Kamonluck Teamtisong: The Center for Scientific and Technological Equipment, Suranaree University of Technology
Panlada Tittabutr: School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Nantakorn Boonkerd: School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Shusei Sato: Graduate School of Life Sciences, Tohoku University
Pakpoom Boonchuen: School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Shin Okazaki: Institute of Global Innovation Research (IGIR), Tokyo University of Agriculture and Technology (TUAT)
Neung Teaumroong: School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology

DOI: https://doi.org/10.1038/s41598-024-75294-4
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 15

Abstract

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Abstract The symbiotic relationship between legumes and rhizobia is known to be influenced by specific rhizobial type III effectors (T3Es) in certain cases. In this study, we present evidence that the symbiosis between Vigna radiata and Bradyrhizobium elkanii USDA61 is controlled by a T3E called NopP2, and this interaction is highly dependent on the genetic makeup of the host plant. NopP2 plays a crucial role in promoting nodulation in various V. radiata varieties. Additionally, NopP2 is essential for early infection and the formation of nodules in compatible plants. Through evolutionary analysis, we discovered that bradyrhizobial NopPs can be categorized into two distinct clusters: NopP1 and NopP2. Furthermore, both types of bradyrhizobial NopPs were conserved within their respective groups. Our findings suggest that NopP2 serves as a mechanism for optimizing the symbiotic relationship between V. radiata and B. elkanii USDA61 by interacting with the pathogenesis related-10 (PR10) protein and reducing effector-triggered immunity (ETI) responses.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

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