Cancer Management and Research (Mar 2021)
miR-621 May Suppress Cell Proliferation via Targeting lncRNA SNHG10 in Acute Myeloid Leukemia
Abstract
Shishan Xiao,1,* Yan Zha,2,* Hongqian Zhu1 1Department of Hematology, Guizhou Provincial People’s Hospital, Guiyang City, Guizhou Province 550002, People’s Republic of China; 2Department of Nephrology, Guizhou Provincial People’s Hospital, Guiyang City, Guizhou Province 550002, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hongqian ZhuGuizhou Provincial People’s Hospital, No. 83 Zhongshan East Road, Guiyang City, Guizhou Province 550002, People’s Republic of ChinaEmail [email protected]: It has been reported that lncRNA SNHG10 (SNHG10) promotes the progression of liver cancer and osteosarcoma; however, the role of SNHG10 in acute myeloid leukemia (AML) remains unknown. This study was to explore the role of SNHG10 in AML.Methods: The expression of SNHG10 and miR-621 in BM mononuclear cells (BMMNCs) isolated from 60 AML patients and 60 healthy controls were determined by RT-qPCR. Correlation between SNHG10 and miR-621 was analyzed by Pearson correlation coefficient. The overexpression of SNHG10 and miR-621 in transfected AML cells was detected by RT-qPCR, and the regulatory relationship between them was explored. Methylation-specific PCR (MSP) was applied to detect the methylation of miR-621 induced by the overexpression of SNHG10. CCK-8 assay was conducted to evaluate cell proliferation.Results: In this study, we found that the expression of SNHG10 was upregulated and the expression of miR-621 was downregulated in AML samples. Moreover, SNHG10 and miR-621 were inversely correlated across AML samples, and a high level of SNHG10 predicted poor survival of AML patients. Bioinformatics analysis showed that SNHG10 could be targeted by miR-621. In AML cells, miR-621 overexpression downregulated the expression of SNHG10, while SNHG10 overexpression could not affect the expression of miR-621. However, it was found that the reduction of miR-621 caused by SNHG10 overexpression might be due to the increase of miR-621 methylation. In addition, SNHG10 overexpression significantly promoted BMMNC proliferation, whereas miR-621 overexpression inhibited BMMNC proliferation and abolished the effect of SNHG10 overexpression on BMMNC proliferation.Conclusion: miR-621 targets SNHG10 to suppress cell proliferation in AML.Keywords: acute myeloid leukemia, miR-621, SNHG10, proliferation
