A thermostable Cas9 with increased lifetime in human plasma

Lucas B. Harrington; David Paez-Espino; Brett T. Staahl; Janice S. Chen; Enbo Ma; Nikos C. Kyrpides; Jennifer A. Doudna

doi:10.1038/s41467-017-01408-4

Nature Communications (Nov 2017)

A thermostable Cas9 with increased lifetime in human plasma

Lucas B. Harrington,
David Paez-Espino,
Brett T. Staahl,
Janice S. Chen,
Enbo Ma,
Nikos C. Kyrpides,
Jennifer A. Doudna

Affiliations

Lucas B. Harrington: Department of Molecular and Cell Biology, University of California
David Paez-Espino: Department of Energy, Joint Genome Institute
Brett T. Staahl: Department of Molecular and Cell Biology, University of California
Janice S. Chen: Department of Molecular and Cell Biology, University of California
Enbo Ma: Department of Molecular and Cell Biology, University of California
Nikos C. Kyrpides: Department of Energy, Joint Genome Institute
Jennifer A. Doudna: Department of Molecular and Cell Biology, University of California

DOI: https://doi.org/10.1038/s41467-017-01408-4
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 8

Abstract

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While current CRISPR-Cas9 tools have revolutionized genome editing, they are not suitable for applications at elevated temperatures. Here, the authors characterize GeoCas9 from Geobacillus stearothermophilus, which is active up to 70°C and is stable in human plasma.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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