Nature Communications (Nov 2017)

A thermostable Cas9 with increased lifetime in human plasma

  • Lucas B. Harrington,
  • David Paez-Espino,
  • Brett T. Staahl,
  • Janice S. Chen,
  • Enbo Ma,
  • Nikos C. Kyrpides,
  • Jennifer A. Doudna

DOI
https://doi.org/10.1038/s41467-017-01408-4
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 8

Abstract

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While current CRISPR-Cas9 tools have revolutionized genome editing, they are not suitable for applications at elevated temperatures. Here, the authors characterize GeoCas9 from Geobacillus stearothermophilus, which is active up to 70°C and is stable in human plasma.