Anti-Inflammatory Triterpene Glycosides from the Roots of Ilex dunniana Levl
Yu-Sheng Shi,
Yan Zhang,
Wen-Zhong Hu,
Xi Chen,
Xin Fu,
Xia Lv,
Li-Hong Zhang,
Ning Zhang,
Guang Li
Affiliations
Yu-Sheng Shi
Key Laboratory of Biotechnology and Bioresources Utilization, Educational of Minister, College of Life Science, Dalian Nationalities University, Dalian 116600, China
Yan Zhang
Jiamusi College, Heilongjiang University of Chinese Medicine, Jiamusi 154007, China
Wen-Zhong Hu
Key Laboratory of Biotechnology and Bioresources Utilization, Educational of Minister, College of Life Science, Dalian Nationalities University, Dalian 116600, China
Xi Chen
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
Xin Fu
Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin 150040, China
Xia Lv
Key Laboratory of Biotechnology and Bioresources Utilization, Educational of Minister, College of Life Science, Dalian Nationalities University, Dalian 116600, China
Li-Hong Zhang
Jiamusi College, Heilongjiang University of Chinese Medicine, Jiamusi 154007, China
Ning Zhang
Jiamusi College, Heilongjiang University of Chinese Medicine, Jiamusi 154007, China
Guang Li
Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jing Hong 666100, China
A new triterpene glycoside ilexdunnoside A (1) and a new sulfated triterpene derivative ilexdunnoside B (2), together with five known analogues 3–7 were isolated from the roots of Ilex dunniana Levl. The structures were established by NMR spectroscopic analysis and acid hydrolysis. Results of an in vivo study of the biological activity showed that 75% ethanol and n-butanol extracts of the plant displayed anti-inflammatory activities against ear edema in mice, with inhibition rates of 23.5% and 37.5%, respectively, at a dose of 50 mg/kg. Furthermore, Compounds 1, 2 and 3 exhibited moderate indirect inhibitory effects on lipopolysaccharide-induced NO production in BV2 microglial cells in vitro, with IC50 values of 11.60, 12.30 and 9.70 μM, respectively.
