Nanoparticles Obtained from Zein for Encapsulation of Mesalazine
Izabela Borges C. Lima,
Lina Clara G. A. I. Moreno,
Ana Victória Peres,
Ana Cristina Gramoza Santana,
Adonias Carvalho,
Mariana H. Chaves,
Lorena Lima,
Rayran Walter Sousa,
Dalton Dittz,
Hercília M. L. Rolim,
Lívio César Cunha Nunes
Affiliations
Izabela Borges C. Lima
Laboratory of Technological Innovation, Entrepreneurship, Medicines and Related (LITE), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Lina Clara G. A. I. Moreno
Pharmaceutical Nanosystems Laboratory (NANOSFAR), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Ana Victória Peres
Laboratory of Technological Innovation, Entrepreneurship, Medicines and Related (LITE), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Ana Cristina Gramoza Santana
Laboratory of Technological Innovation, Entrepreneurship, Medicines and Related (LITE), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Adonias Carvalho
Natural Products Laboratory (LPN), Department of Chemistry, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Mariana H. Chaves
Natural Products Laboratory (LPN), Department of Chemistry, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Lorena Lima
Laboratory of Technological Innovation, Entrepreneurship, Medicines and Related (LITE), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Rayran Walter Sousa
Laboratory of Experimental Cancerology (LabCâncer), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Dalton Dittz
Laboratory of Experimental Cancerology (LabCâncer), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Hercília M. L. Rolim
Pharmaceutical Nanosystems Laboratory (NANOSFAR), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, Brazil
Lívio César Cunha Nunes
Laboratory of Technological Innovation, Entrepreneurship, Medicines and Related (LITE), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, Brazil
We encapsulated MSZ in zein nanoparticles (NP-ZN) using a desolvation method followed by drying in a mini spray dryer. These nanoparticles exhibited a size of 266.6 ± 52 nm, IPD of 0.14 ± 1.1 and zeta potential of −36.4 ± 1.5 mV, suggesting colloidal stability. Quantification using HPLC showed a drug-loaded of 43.8 µg/mg. SEM demonstrated a spherical morphology with a size variation from 220 to 400 nm. A FTIR analysis did not show drug spectra in the NPs in relation to the physical mixture, which suggests drug encapsulation without changing its chemical structure. A TGA analysis showed thermal stability up to 300 °C. In vitro release studies demonstrated gastroresistance and a sustained drug release at pH 7.4 (97.67 ± 0.32%) in 120 h. The kinetic model used for the release of MSZ from the NP-ZN in a pH 1.2 medium was the Fickian diffusion, in a pH 6.8 medium it was the Peppas–Sahlin model with the polymeric relaxation mechanism and in a pH 7.4 medium it was the Korsmeyer–Peppas model with the Fickian release mechanism, or “Case I”. An in vitro cytotoxicity study in the CT26.WT cell line showed no basal cytotoxicity up to 500 μg/mL. The NP-ZN showed to be a promising vector for the sustained release of MSZ in the colon by oral route.
