Visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissues
Chenyang Hao,
Wei Yang,
Gangqiang Dong,
Yuetong Yu,
Yan Liu,
Jun Zhang,
Yongping Zhu,
Xiaolu Wei,
Sha Chen
Affiliations
Chenyang Hao
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China
Wei Yang
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China
Gangqiang Dong
Amway (China) Botanical R&D Center, Wuxi 214115, China
Yuetong Yu
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China
Yan Liu
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China
Jun Zhang
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China
Yongping Zhu
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China
Xiaolu Wei
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China; Corresponding author.
Sha Chen
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China; Corresponding author.
Benzylisoquinoline alkaloids in lotus (Nelumbo nucifera) seed plumules and leaves exhibit significant tissue specificity for their pharmacological effects and potential nutritional properties. Herein, 46 benzylisoquinoline alkaloids were identified via UPLC-QTOF-HRMS, of which 9 were annotated as glycosylated monobenzylisoquinoline alkaloids concentrated in the seed plumules. The spatial distribution of targeted benzylisoquinoline alkaloids in leaves, seed plumules, and milky sap was determined via MALDI–MSI. Furthermore, 37 Nelumbo cultivars were investigated using targeted metabolomics to provide insights into functional tea development. While aporphine alkaloids comprised the main compounds present in lotus leaves, bisbenzylisoquinoline alkaloids were the main compounds in lotus plumules, where glycosylation primarily occurs. These findings can help understand the distribution of benzylisoquinoline alkaloids in lotus tissue and the directional breeding of varieties enriched with specific chemical functional groups for nutritional and pharmacological applications.
