Gene expression of P53 and adipoq as diagnostic markers for colorectal cancer

Abstract

Purpose: Colorectal cancer is the most frequent cause of death and had high mortality rate in Western world. It is from complex, variable and patient- specific interaction between genetic, epigenetic and environmental factors. In the present study, we aimed to investigate the contribution of gene expression of the P53 and Adipoq, both genes to the risk of colorectal cancer. P53 gene is a tumor suppressor gene, encoded protein of P53 is a transcription factor and its pivotal role in maintaining genomic stability. Adipoq gene codes adiponectin. Material and Methods: Total RNA were extracted from paired tumor and normal tissues of 32 colorectal cancer patients. The mRNA expression level of P53 and Adipoq were measured employing semi- quantitative reverse transcription- polymerase chain reaction (RT- PCR). Results: The mRNA expression level of P53 in colorectal cancer was significantly increased according to normal samples (over-expressed). However, the mRNA expression level of Adipoq in colorectal cancer was significantly reduced according to normal samples (down- regulated). Conclusion: In current study, our data suggest those reduced mRNA expression of the Adipoq and increased mRNA expression of P53 might be useful molecular diagnostic markers for colorectal cancer patients. In order to understand the investigation between colorectal cancer and diagnostic biomarker; further analysis is necessary. [Cukurova Med J 2016; 41(2.000): 217-223]

Keywords

• Key words: Colorectal cancer
• P53
• Adipoq
• mRNA expression analysis
• semi- quantitative RT- PCR