Nature Communications (Feb 2020)
A conserved strategy of chalcone isomerase-like protein to rectify promiscuous chalcone synthase specificity
- Toshiyuki Waki,
- Ryo Mameda,
- Takuya Nakano,
- Sayumi Yamada,
- Miho Terashita,
- Keisuke Ito,
- Natsuki Tenma,
- Yanbing Li,
- Naoto Fujino,
- Kaichi Uno,
- Satoshi Yamashita,
- Yuichi Aoki,
- Konstantin Denessiouk,
- Yosuke Kawai,
- Satoko Sugawara,
- Kazuki Saito,
- Keiko Yonekura-Sakakibara,
- Yasumasa Morita,
- Atsushi Hoshino,
- Seiji Takahashi,
- Toru Nakayama
Affiliations
- Toshiyuki Waki
- Graduate School of Engineering, Tohoku University
- Ryo Mameda
- Graduate School of Engineering, Tohoku University
- Takuya Nakano
- Graduate School of Engineering, Tohoku University
- Sayumi Yamada
- Graduate School of Engineering, Tohoku University
- Miho Terashita
- Graduate School of Engineering, Tohoku University
- Keisuke Ito
- Graduate School of Engineering, Tohoku University
- Natsuki Tenma
- Graduate School of Engineering, Tohoku University
- Yanbing Li
- Graduate School of Engineering, Tohoku University
- Naoto Fujino
- Graduate School of Engineering, Tohoku University
- Kaichi Uno
- Graduate School of Engineering, Tohoku University
- Satoshi Yamashita
- Graduate School of Natural Science and Technology, Kanazawa University
- Yuichi Aoki
- Department of Integrative Genomics, Tohoku Medical Megabank Organization
- Konstantin Denessiouk
- Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University
- Yosuke Kawai
- Genome Medical Science Project, National Center for Global Health and Medicine
- Satoko Sugawara
- The RIKEN Center for Sustainable Resource Science
- Kazuki Saito
- The RIKEN Center for Sustainable Resource Science
- Keiko Yonekura-Sakakibara
- The RIKEN Center for Sustainable Resource Science
- Yasumasa Morita
- Experimental Farm, Faculty of Agriculture, Meijo University
- Atsushi Hoshino
- National Institute for Basic Biology
- Seiji Takahashi
- Graduate School of Engineering, Tohoku University
- Toru Nakayama
- Graduate School of Engineering, Tohoku University
- DOI
- https://doi.org/10.1038/s41467-020-14558-9
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 14
Abstract
Chalcone synthase is the first committed enzyme in the plant flavonoid biosynthesis pathway, yet shows low product specificity in vitro. Here Waki et al. show that chalcone isomerase-like proteins bind to and reduce the catalytic promiscuity of chalcone synthase, ensuring efficient flavonoid production in planta.
