DNA-free RNA preparations from mycobacteria

Abstract

Abstract Background To understand mycobacterial pathogenesis analysis of gene expression by quantification of RNA levels becomes increasingly important. However, current preparation methods yield mycobacterial RNA that is contaminated with chromosomal DNA. Results After sonication of RNA samples from Mycobacterium smegmatis genomic DNA is efficiently removed by DNaseI in contrast to untreated samples. Conclusions This procedure eliminates one of the most prevalent error sources in quantification of RNA levels in mycobacteria.