Nature-inspired topographies on hydroxyapatite surfaces regulate stem cells behaviour

Yogambha Ramaswamy; Iman Roohani; Young Jung No; Genevieve Madafiglio; Frank Chang; Furong Zhao; Zufu Lu; Hala Zreiqat

Bioactive Materials (Apr 2021)

Nature-inspired topographies on hydroxyapatite surfaces regulate stem cells behaviour

Yogambha Ramaswamy,
Iman Roohani,
Young Jung No,
Genevieve Madafiglio,
Frank Chang,
Furong Zhao,
Zufu Lu,
Hala Zreiqat

Affiliations

Yogambha Ramaswamy: Biomaterials and Tissue Engineering Research Unit, School of Biomedical Engineering, University of Sydney, Sydney, NSW, 2006, Australia; Australian Research Centre for Innovative BioEngineering, University of Sydney, Sydney, NSW, 2006, Australia
Iman Roohani: School of Chemistry, Australian Centre for Nanomedicine, University of New South Wales, Sydney NSW, Australia; Corresponding author.
Young Jung No: Biomaterials and Tissue Engineering Research Unit, School of Biomedical Engineering, University of Sydney, Sydney, NSW, 2006, Australia; Australian Research Centre for Innovative BioEngineering, University of Sydney, Sydney, NSW, 2006, Australia
Genevieve Madafiglio: Biomaterials and Tissue Engineering Research Unit, School of Biomedical Engineering, University of Sydney, Sydney, NSW, 2006, Australia
Frank Chang: Biomaterials and Tissue Engineering Research Unit, School of Biomedical Engineering, University of Sydney, Sydney, NSW, 2006, Australia
Furong Zhao: Biomaterials and Tissue Engineering Research Unit, School of Biomedical Engineering, University of Sydney, Sydney, NSW, 2006, Australia
Zufu Lu: Biomaterials and Tissue Engineering Research Unit, School of Biomedical Engineering, University of Sydney, Sydney, NSW, 2006, Australia; Australian Research Centre for Innovative BioEngineering, University of Sydney, Sydney, NSW, 2006, Australia
Hala Zreiqat: Biomaterials and Tissue Engineering Research Unit, School of Biomedical Engineering, University of Sydney, Sydney, NSW, 2006, Australia; Australian Research Centre for Innovative BioEngineering, University of Sydney, Sydney, NSW, 2006, Australia; Corresponding author. Biomaterials and Tissue Engineering Research Unit, School of Biomedical Engineering, University of Sydney, Sydney, NSW, 2006, Australia.

Journal volume & issue: Vol. 6, no. 4
pp. 1107 – 1117

Abstract

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Surface topography is one of the key factors in regulating interactions between materials and cells. While topographies presented to cells in vivo are non-symmetrical and in complex shapes, current fabrication techniques are limited to replicate these complex geometries. In this study, we developed a microcasting technique and successfully produced imprinted hydroxyapatite (HAp) surfaces with nature-inspired (honeycomb, pillars, and isolated islands) topographies. The in vitro biological performance of the developed non-symmetrical topographies was evaluated using adipose-derived stem cells (ADSCs). We demonstrated that ADSCs cultured on all HAp surfaces, except honeycomb patterns, presented well-defined stress fibers and expressed focal adhesion protein (paxillin) molecules. Isolated islands topographies significantly promoted osteogenic differentiation of ADSCs with increased alkaline phosphatase activity and upregulation of key osteogenic markers, compared to the other topographies and the control unmodified (flat) HAp surface. In contrast, honeycomb topographies hampered the ability of the ADSCs to proliferate and differentiate to the osteogenic lineage. This work presents a facile technique to imprint nature-derived topographies on the surface of bioceramics which opens up opportunities for the development of bioresponsive interfaces in tissue engineering and regenerative medicine.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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