Cancer Management and Research (Jun 2021)
LINC-PINT Inhibited Malignant Progression of Bladder Cancer by Targeting miR-155-5p
Abstract
Xiancheng Han,1 Jing Liu,2 Yongguo Liu,1 Linkai Mou,1 Chunlong Li1 1Department of Urology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, 261031, People’s Republic of China; 2Department of Pathology, Weifang Traditional Chinese Hospital, Weifang, Shandong, 261041, People’s Republic of ChinaCorrespondence: Yongguo LiuDepartment of Urology, Affiliated Hospital of Weifang Medical University, No. 2428, Yuhe Road, Weifang, Shandong, 261031, People’s Republic of China, Tel +86-0536-3081339Email [email protected]: This study mainly explored the expression level of LINC-PINT in bladder cancer and its relationship with prognosis. Meanwhile, the effect of LINC-PINT on the biological function of bladder cancer was also explored.Methods: The expression levels of LINC-PINT and miR-155-5p were detected by qRT-PCR. The prognostic significance of LINC-PINT in bladder cancer was studied by the Kaplan–Meier curve and Log rank test. CCK-8 and Transwell assays were used to analyze the proliferation, migration, and invasion ability. The targeting relationship between LINC-PINT and miR-155-5p was analyzed using bioinformatics and dual-luciferase reporter assays.Results: The expression of LINC-PINT was downregulated in bladder cancer tissues and cell lines, and miR-155-5p showed the opposite trend in bladder cancer tissues. Kaplan–Meier curve proved that the patients with low LINC-PINT expression had a lower five-year survival rate and the Log rank test displayed that LINC-PINT was a prognostic factor of BC. CCK-8 and Transwell results showed that LINC-PINT could inhibit the ability of proliferation, migration, and invasion. LINC-PINT was proved to target miR-155-5p in bladder cancer. Dual-luciferase reporter gene assay showed that the relative luciferase activity of overexpression miR-155-5p co-transfected with LINC-PINT-wt was significantly lower. LINC-PINT was negatively correlated with miR-155-5p.Conclusion: LINC-PINT is a potential prognostic marker of bladder cancer, and the up-regulation of Lin-PINT can inhibit the proliferation, invasion, and migration of bladder cancer cells by targeting miR-155-5p.Keywords: LINC-PINT, bladder cancer, miR-155-5p, inhibited, progression
