The expression of the miR-193, miR-122 and miR-155 profiling and evaluation the serum lipid in antimony-susceptible and resistance patients with cutaneous leishmaniasis

Abstract

Background & objectives: The current investigation was carried out to evaluate the expression of MicroRNAs miR-193, miR-122 and miR-155 and lipid profile in antimony-susceptible and resistance patients with cutaneous leishmaniasis. Methods: Lesion and blood samples were collected from 27 antimony-resistance and 27 antimony-susceptible patients. mRNA was extracted and synthase to the cDNA using commercial kits according to the manufacturers’ guideline. The expression of miR-193, miR-122 and miR-155 were evaluated using Real-Time PCR technique. The serum lipid profiles were measured by enzymatic methods. Results: Our results indicated that the expression of miR-193, miR-122 and miR-155 was significantly higher in antimony-susceptible patients. The results of current study indicated that downregulation of miRNAs is coupled with low serum LDL-C and triglyceride. Interpretation & conclusion: The downregulation of miRNAs and decrease in lipid levels may be one of the mechanisms of the parasite to escape from host immune system.

Keywords

• cutaneous leishmaniasis
• mir-122
• mir-193
• mir-155
• drug resistance